用核酸序列测定1型糖尿病HLA-DPB1和DQB1第2外显子基因

目的 研究山东省汉族1型糖尿病与HLA－DPB1和HLA－DQB1等位基因的相关性。方法 采用基于核酸序列测定的基因分型技术对52例1型糖尿病患者及38例正常对照进行了DPB1和DQB1基因分析。结果 DPB1＊2201（P＜0．01）和DQB1＊0201（P＜0．01）、＊0303（P＜0．05）及＊0604（P＜0．05）等位基因频率在糖尿病患者组显著高于对照组，而PB1＊0402（P＜0．01）和DQB1＊0301（P＜0．01）等位基因在糖尿病患者组显著低于对照组。结论 DPB1＊2201和DQB1＊0201、＊0303及＊0604等位基因可能是山东省汉族1型糖尿病的易感性等位基因，而DPB1＊0402和DQB1＊0301等位基因可能是山东省汉族1型糖尿病的保护性等位基因。

Sequencing-based typing of HLA-DPB1 and DQB1 exon 2 in patients with type 1 diabetes mellitus

Objective To investigate the correlation betwee n type 1 diabetes mellitus susceptibility and HLA-DPB1 and DQB1 genes in Shandong Han population.Methods HLA-DPB1 and DQB1 genes analysis were performed by sequencing-based typing in 52 type 1 diabetes mellitus patients an d 38 normal controls.Results The frequency of DPB1*2201 in diabetics w as significantly higher than that in controls(26.92% vs 5.26%, P<0 01) , b ut t he frequency of DPB1*0402 in diabetics was significantly lower than that in cont rols(11.54% vs 34.21%, P<0 01) . The frequencies of DQB1*0201, *0303 and *0604 in diabetics were higher than those in controls (24.04% vs 10.53%, P <0.0 1; 22.12% vs 6.58%, P<0 05 ; 19.23% vs 7.89%, P<0.05) , while the f requency of DQB1*0301 in diabetics was significantly lower than that in controls (4.81% vs 25.00%, P<0 01) . Conclusion DPB1*2201, DQB1*0201, *0303 and *0604 may be susceptible alleles, and DPB1*0402 and DQB1*0301 may be protective alleles in patients with type 1 diabetes mellitus.