| 碘缺乏和甲状腺功能减退对大鼠仔鼠海马钙调蛋白表达的影响 |
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| 引用本文： | 董静,刘万洋,王毅,魏薇,陈杰. 碘缺乏和甲状腺功能减退对大鼠仔鼠海马钙调蛋白表达的影响[J]. 毒理学杂志, 2010, 0(2) |
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| 作者姓名： | 董静 刘万洋 王毅 魏薇 陈杰 |
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| 作者单位： | 中国医科大学公共卫生学院劳动卫生与环境卫生学科; |
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| 基金项目： | 国家自然科学基金项目(30500400) |
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| 摘 要： | 目的观察碘缺乏和甲状腺功能减退对大鼠仔鼠海马钙调蛋白(calmodulin)表达的影响。方法健康2月龄雌性Wistar大鼠,交配妊娠后,取孕鼠28只,按体重随机分成对照组、碘缺乏组、甲状腺功能减退1和2组。自怀孕第6天(gestational day6,GD6)起,碘缺乏组饲以缺碘地区粮食配制的饲料,饮用自来水;甲状腺功能减退1和2组分别给予5和15ppm丙基硫尿嘧啶(propylthiouracil,PTU)饮水,饲以普通饲料,至生后第28天(postnatal day28,PN28)。分别于PN7、PN14和PN21时,每组随机取5只仔鼠,灌流固定大脑,进行组织病理切片和免疫组化染色,观察分析海马钙调蛋白表达。结果PN14和PN21时,在CA1和CA3区,15ppm组和碘缺乏组仔鼠海马钙调蛋白表达显著低于对照组(P0.05)。在DG区,钙调蛋白与对照组相比,差异无统计学意义。PN7时,各区均几乎观察不到阳性反应产物,各组间蛋白表达差异无统计学意义。结论在本试验条件下,碘缺乏和甲状腺功能减退可下调海马CA1和CA3区钙调蛋白蛋白表达。
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| 关 键 词： | 碘缺乏 甲状腺功能减退 海马 钙调蛋白 |
Effects of iodine deficiency and hypothyroidism on the expression of calmodulin in hippocampus of pups |
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| DONG Jing,LIU Wan-yang,WANG Yi,WEI Wei,CHEN Jie. Effects of iodine deficiency and hypothyroidism on the expression of calmodulin in hippocampus of pups[J]. Journal of Toxicology, 2010, 0(2) |
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| Authors: | DONG Jing LIU Wan-yang WANG Yi WEI Wei CHEN Jie |
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| Affiliation: | DONG Jing,LIU Wan-yang,WANG Yi,WEI Wei,CHEN Jie (Department of Occupational , Environmental Health,School of Public Health,China Medical University,Shenyang Liaoning 110001,China) |
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| Abstract: | Objective To observe the effects of iodine deficiency and hypothyroidism on the expression of calmodulin in hippocampus of pups. Methods Female Wistar rats ( n = 28) after pregnancy were randomly divided into control group, hypothyroid group 1 and 2 and iodine-deficient group (7 in each group). From gestational day ( GD) 6 to postnatal day ( PN) 28,iodine-deficient group was administered with iodine-deficient diet and tap water; hypothyroid group 1 and 2 were administered 5 ppm and 15 ppm propylthiouracil (... |
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| Keywords: | Iodine deficiency Hypothyroidism Hippocampus Calmodulin |
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