急性暴露尼古丁对未成年大鼠脑内神经元Caspase-3表达及凋亡的影响

目的研究急性暴露尼古丁（nicotine）对未成年大鼠脑内神经细胞凋亡的影响。方法用免疫组织化学法和Westem blotting法检测急性暴露尼古丁的未成年模型大鼠脑内Caspase-3的表达；用原位末端标记法（TUNEL法）检测凋亡细胞。结果免疫组织化学法检测到大脑皮层、海马齿状回、CA2区和CA3区的Caspase-3表达在实验组明显增高。蛋白质免疫印迹法（Western blotting）检测到大脑皮层和海马Caspase-3原型及活性片段的表达在实验组明显增高。TUNEL法检测到大脑皮层和海马齿状回的阳性细胞在实验组明显增多。结论急性暴露尼古丁激活未成年大鼠脑内大脑皮层和海马内神经细胞Caspase-3，促进大脑皮层和海马齿状回的神经元凋亡。

EFFECTS OF ACUTE NICOTINE EXPOSURE ON CASPASE-3 EXPRESSION AND APOPTOSIS OF NEURON IN THE ADOLESCENT RAT'S BRAIN

Objective To investigate the effects of acute nicotine exposure on expression of Caspase-3 and apoptosis of neuron in adolescent rat's brain. Methods The Caspase-3 expression was detected with immunohistochemistry and Western blotting. Apoptosis cells were detected by TUNEL method. Results The Caspase-3 expression in cerebral cortex, hippocampal dentate gyrus, CA2 and CA3 of the experimental group was higher than that of the control group through immunohistiochemistry. The expression of Caspase-3 prototype and active fragments in cerebral cortex and hippocampus of the experimental group was higher than that of the control group with Western blotting. The TUNEL positive cells in cerebral cortex and hippocampal dentate gyrus of the experimental group were more than that of the control group.Conclusion Acute nicotine exposure can activate Caspase-3 and increase neuron apoptosis in cerebral cortex and hippocampal dentate gyrus of the adolescent rat's brain.