| C75对肝癌细胞侵袭转移的影响及其机制 |
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| 引用本文： | 王益东,陈昆仑,朱海涛,刘仲伟,刘妮,徐心.C75对肝癌细胞侵袭转移的影响及其机制[J].山西医学院学报,2013(10):781-784,833. |
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| 作者姓名： | 王益东 陈昆仑 朱海涛 刘仲伟 刘妮 徐心 |
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| 作者单位： | [1]西安交通大学医学院第二附属医院普通外科,西安710004 [2]西安交通大学医学院,西安710004 |
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| 基金项目： | 陕西省科技攻关计划基金资助项目[2010k14-02（20）] |
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| 摘 要： | 目的 观察C75对肝癌MHCC97H细胞侵袭转移的作用,并探讨其作用机制. 方法 通过划痕实验和Transwell小室检测C75对肝癌MHCC97H细胞迁徙和侵袭的影响.采用Western blot的方法探讨C75对MMP-2、MMP-9及其抑制物TIMP-2、TIMP-1表达的影响.采用明胶酶谱的方法检测C75对MMP-2和MMP-9蛋白酶活性的影响. 结果 C75能够明显抑制MHCC97H细胞迁徙能力和侵袭能力;C75能够抑制MHCC97H细胞中MMP-2、MMP-9的表达水平及酶活性,且能够增高TIMP-1和TIMP-2的表达水平. 结论 C75能够抑制肝癌细胞MHCC97H的迁徙和侵袭能力.这种抑制作用可能与其对MMP/TIMP的平衡调节相关.
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| 关 键 词： | C75 肝癌 侵袭 基质金属蛋白酶 |
Effect of C75 on invasion and migration of hepatic cancer cells and its mechanism |
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| Authors: | WANG Yidong CHEN Kunlun ZHU Haitao LIU Zhongwei LIU Ni XU Xin |
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| Institution: | 1.Department of General Surgery,Second Affiliated Hospital,Medical School of Xi＇an Jiaotong University, Xi＇an 710004, China;Medical School of Xi＇an Jiaotong University;) |
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| Abstract: | Objective To investigate the effect of a FASN inhibitor C75 on MHCC97H cell invasion and migration,and to explore its molecular mechanisms.Methods The anti-metastatic effect of C75 was determined using wound healing assay and Transwell invasion model.The expression of MMP-2,MMP-9,TIMP-1 and TIMp-2 protein in MHCC97H cells was determined by Western blot.The activities of MMP-2 and MMP-9 were determined by gelatin-zymography.Results The migration and invasion of MHCC97H cells were markedly suppressed by C75 in a dose-dependent manner.After treatment with C75 for 24 h,the expression of MMP-2 and MMP-9,and proteinase activities decreased.Meanwhile,the expression of TIMP-1 and TIMP-2 were increased in a dose-dependent fashion.Conclusion These findings suggest that C75 preferentially inhibits HCC invasion by regulating the synthesis of proteinases and their inhibitors.C75 may be a potential novel therapeutic agent for HCC. |
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| Keywords: | C75 hepatocellular carcinoma invasion MMP |
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