白芍总苷对卡介苗加脂多糖引起的小鼠免疫性肝损伤的保护作用

目的　研究白芍总苷对小鼠免疫性肝损伤的保护作用。方法　在建立BCG +LPS诱导免疫性肝损伤小鼠模型的基础上 ,分光光度法检测血清中ALT、AST、NO水平和肝匀浆MDA、GSH Px、SOD含量 ;放免法检测TNF α的生物学活性 ;细胞增殖法测定脾淋巴增殖反应。结果　白芍总苷 (6 0、12 0、2 4 0mg·kg-1)ig给药可明显降低免疫性肝损伤小鼠增高的血清ALT、AST活性 ,同时能减少肝匀浆MDA含量 ,使降低的肝匀浆GSH Px、SOD活性升高 ,进一步研究发现白芍总苷可明显抑制免疫性肝损伤小鼠血清NO和TNF α的产生。白芍总苷还可抑制小鼠腹腔巨噬细胞TNF α的产生 ,对ConA诱导的脾淋巴增殖反应具有恢复作用 ,而对LPS诱导的脾淋巴增殖反应无明显影响。结论　白芍总苷对免疫性肝损伤具有保护作用。

Protective effect of total glucosides of paeony on immunological liver injury in mice induced by Bacillus Calmette-Guerin plus lipopolysaccharide

Aim To investigate the prophylactic effect of total glucosides of paeony (TGP) on immunological liver injury in mice. Methods The model of immunological liver injury in mice was produced by injection of Bacillus Calmette Guerin (BCG) and lipopolysaccharide(LPS) from tail vein. The alanine aminotransferase(ALT), aspartate aminotransferase(AST), nitric oxide (NO) level in serum and malondiadehyde(MDA) content, superoxide dismutase(SOD) and glutathione peroxidase(GSH Px) activities in liver homogenate were assayed by spectrophotometry; Tumour necrosis factor α (TNF α) activity was determined by radio immunoassay method. ConA and LPS induced splenocytes proliferation was assayed by method of cell proliferation. Results TGP(60,120,240 mg·kg -1 ) was found to significantly decrease the serum transaminase activities. Meanwhile, TGP also decreased MDA content and prevented the reduction of SOD and GSH Px activities in liver homogenate. TGP significantly reduced TNF α and NO levels in serum Furthermore, TGP was shown to inhibit TNF α production by peritoneal macrophages and reverse ConA induced splenocyte proliferation response, whereas TGP showed no effect on LPS induced splenocyte proliferation response. Conclusion TGP showed significant protective action on immunological liver injury in mice.