化疗对大鼠脑胶质瘤自由基清除作用的影响

目的:通过观察联合化疗对胶质瘤细胞的作用,分析肿瘤细胞和脑皮质的MDA含量及SOD活性,阐述自由基在联合化疗中对胶质瘤的抑制作用。方法: 利用体外C₆胶质瘤细胞培养,测定化疗前后C₆细胞上清中MDA含量和SOD活性的变化。测定化疗前后,瘤鼠脑皮质中MDA含量及SOD活性的变化。 结果: 化疗可使C₆胶质瘤细胞上清各组MDA含量均降低,其中以3种药物联合组(B+V+N)MDA含量降低最为显著;另外,SOD活性也降低,2种药物联合组(B+N,V+N)和3种药物联合组(B+V+N)均低于对照组。化疗使肿瘤鼠脑皮质各组MDA含量均降低;脑皮质各组SOD活性均升高,以B+V+D+N+A₂组最明显。联合化疗加压组(B+V+D+N+A₂)抑瘤率高于联合化疗不加压组(B+V+D+N)。联合化疗组肿瘤鼠生存期长于单独化疗组(B+N)。 结论: 化疗的抑瘤作用机制可通过抑制脂质过氧化反应,清除自由基来实现。

Effects of chemotherapy on free radicals in the rat glioma

AIM: To observe the role of free radicals in the inhibitory effect of chemotherapy on glioma cells. METHODS: C6 glioma cells were cultured in vitro, and treated with carmustine (B), teniposide (V), or/and nimodipine (N). Furthermore, the glioma-bearing rats were treated with B plus N, B+V+lisplatin (D)+N, or B+V+D+N+angiotensin Ⅱ. The MDA content and superoxide dismutase (SOD) activity in the culture supernatant and cortical brain tissue were assayed. RESULTS: B, V and N significantly decreased MDA content and SOD activity in the supernatant of glioma cell culture and C6 glioma cells. Chemotherapy reduced MDA content and increased SOD activity in the cortical brain tissue of tumor-bearing rats, with highest efficiency in B+V+D+N+angiotensin Ⅱ group. The survival time of tumor-bearing rats in B+V+D+N+angiotensin Ⅱ group was longer than that in other chemotherapy group. CONCLUSION: The antitumor effects of combined chemotherapy may be involved in the free radical metabolism.