肠必安治疗免疫致敏法致大鼠溃疡性结肠炎的实验研究

目的：探讨肠必安治疗大鼠溃疡性结肠炎（UC）的作用机理。方法：采取免疫致敏法制备模型，运用完全随机设计，将实验大鼠随机分为5纽，分别为：正常对照组、模型对照组、阳性（锡类散）对照组、肠必安低剂量组、肠必安高剂量组，并给予相应药物灌肠，以各组实验大鼠处理前后体重变化、结肠黏膜损伤度、溃疡面积、组织病理学改变、大便成形时间、胸腺及脾脏系数、白介素-6（IL-6）、C-反应蛋白（CRP）等为检测指标，对其疗效及机理进行研究。结果：治疗后肠必安组的体重变化、结肠黏膜损伤度、溃疡面积、组织病理学的改变、CRP值方面与模型对照组相比有显著性差异（P〈0．05）；肠必安组在结肠黏膜损伤度、溃疡面积、组织病理学改变方面与阳性对照组相比有显著性差异（P〈0．05）。结论：肠必安与锡类散对溃疡性结肠炎均有治疗作用，但在消除炎症反应，修复结肠黏膜及改善肠功能等方面，肠必安优于锡类散；肠必安高剂量组的结肠黏膜损伤度略优于低剂量组；IL-6作为炎症反应程度及抗炎药物疗效的检测指标较CRP更灵敏、更准确。

Experimental Study of Chang-bi-An on Ulcerative Colitis Loaded by Rats of Immune Sensitization

Objective：To investigate the mechanism of action of Chang-bi-An on ulcerative colitis. Method：After preparing models by immune sensitization, the experimental rats were randomly divided into 5 groups, namely： normal control group, model control group ,positive （Xi-lei-san） control group, Chang-bi-An high-dose group, and Chang-bi-An low-dose group. Then the appropriate drugs were given to rats in each group by enema. Such data were detected as weight change before and after treating, colon mucosal damage degrees, the ulcer area, histopatho- logical changes, stool forming time, thymus and spleen coefficients, white Interleukin -6 （ IL-6 ）, C-reactive protein （CRP） ,etc. After statistical processing efficacy and mechanism were observed and studied. Result： After treatment there were significant difference between Chang-bi-An groups and model control group on weight change, colon mucosal damage degrees, ulcer size, histopathological changes, and CRP values （ P 〈 0.05 ）. And there was also significant difference between Chang-bi-An groups and positive （Xi-lei-san） control group on degree of colon mucosal damage, ulcer size, and histopathological changes （ P 〈 0.05 ）. Conclusion ： Chang-bi-An and Xi-lei-san both have a therapeutic effect on ulcerative colitis. But on elimination of inflammation, repairation of colonic mucosa and to improvement of intestinal functions, Chang-bi-An is better than Xi-lei-san. The degree of colonic mucosal damage in Chang-bi-An high-dose group is slightly better than low-dose group, and IL-6, as the extent of inflammatory response and data of anti-inflammatory drug, is more sensitive and more accurate than CRP.