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| CTLA4-Ig基因修饰的树突状细胞体外对Th1/Th2平衡的影响 | |
| 引用本文： | 应林燕,符州,罗健,陈艳,刘恩梅,王莉佳. CTLA4-Ig基因修饰的树突状细胞体外对Th1/Th2平衡的影响[J]. 细胞与分子免疫学杂志, 2011, 27(3) |
| 作者姓名： | 应林燕 符州 罗健 陈艳 刘恩梅 王莉佳 |
| 作者单位： | 1. 重庆医科大学附属儿童医院呼吸中心,重庆,400014;儿童发育疾病研究省部共建教育部重点实验室,重庆,400014 2. 重庆医科大学附属儿童医院呼吸中心,重庆,400014 3. 上海复但大学附属儿科医院,上海,201102 4. 儿童发育疾病研究省部共建教育部重点实验室,重庆,400014 |
| 基金项目： | 国家自然科学基金资助项目(30600684) |
| 摘 要： | 目的:探讨细胞毒性T淋巴细胞相关抗原4免疫球蛋白(CTLA4 Ig)基因修饰的树突状细胞(CTLA4 Ig-DCs)体外对Th1/Th2平衡的影响。方法:通过腺病毒载体将目的基因(CTLA4 Ig)转染至小鼠骨髓来源的树突状细胞(DC)。采用流式细胞术(FCM)检测DC表面分子和胞内CTLA4 Ig的表达;采用混合淋巴细胞反应检测DC刺激同种异体T细胞的能力,ELISA法检测DC抗原提呈反应中Th1和Th2类细胞因子IFN-γ和IL-4分泌水平。结果:CTLA4 Ig基因成功转染至DC,转染率约为80%,制备的CTLA4 Ig-DCs稳定表达CTLA4Ig,表面分子CD86呈现低表达;CTLA4 Ig-DCs可有效抑制T细胞增殖,降低抗原提呈反应上清中IFN-γ和IL-4的分泌,并增加IFN-γ/IL-4比值。结论:通过腺病毒将CTLA4 Ig转染DC并且高效表达,可有效降低DC表面CD86分子,抑制同种异体T细胞反应,并能影响体外Th1/Th2水平。 |
| 关 键 词： | 树突状细胞 CTLA4Ig Th1/Th2平衡 |
The effect of CTLA4-Ig gene modified dendritic cell on Th1/Th2 balance in vitro |
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| YING Lin-yan,FU Zhou,LUO Jian,CHEN Yan,LIU En-mei,WANG Li-jia. The effect of CTLA4-Ig gene modified dendritic cell on Th1/Th2 balance in vitro[J]. Chinese journal of cellular and molecular immunology, 2011, 27(3) | |
| Authors: | YING Lin-yan FU Zhou LUO Jian CHEN Yan LIU En-mei WANG Li-jia |
| Affiliation: | YING Lin-yan1,FU Zhou1,LUO Jian1,CHEN Yan2,LIU En-mei1,WANG Li-jia1 1Department of Respiratory Center,Children's Hospital,Chongqing Medical University,Chongqing 400014,2Children's Hospital of Fudan University,Shanghai 201102,China |
| Abstract: | AIM: To investigate the impact on Th1/Th2 subset balance from cytotoxic T lymphocyte-associated antigen immunoglobulin(CTLA4 Ig) gene-modified dendritic cells(CTLA4 Ig-DCs) in vitro.METHODS: The modified DCs(CTIA4 Ig-DCs) were prepared by transferring the mouse bone marrow-derived DCs with the constructed adenovirus CTIA4 Ig vector.The CTLA4 Ig expression and certain cell surface molecules on the CTIA4Ig-DCs were detected by FCM,the potential to stimulate allogeneic T cell proliferation of the modified DCs ... |
| Keywords: | dendritic cell CTLA4 Ig Th1/Th2 balance |
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