首页 | 本学科首页   官方微博 | 高级检索  
     

全反式维甲酸诱导中脑神经干细胞向多巴胺能神经元分化的作用
引用本文:盛汉松,张弩,林逢春,周辉,林坚,尹波. 全反式维甲酸诱导中脑神经干细胞向多巴胺能神经元分化的作用[J]. 温州医学院学报, 2011, 41(1): 6-9,12
作者姓名:盛汉松  张弩  林逢春  周辉  林坚  尹波
作者单位:温州医学院附属第二医院,神经外科,浙江,温州,325027
基金项目:浙江省科技厅科研基金资助项目(2007C23026); 浙江省卫生厅科研基金资助项目(2007B146); 温州市科技局科研基金资助项目(Y20070042)
摘    要:目的:观察全反式维甲酸(all-trans retinoic acid,ATRA)体外对大鼠中脑神经干细胞(neural stem cells,NSCs)的诱导分化作用及其可能的分子机制。方法:分离培养孕14~15 d大鼠中脑NSCs,使用1.0μmol/L ATRA诱导1、3、5和7 d,以免疫组织化学染色观察ATRA对NSCs分化为多巴胺能神经元的影响;RT-PCR分析维甲酸(retiadic acid,RA)各受体表达情况。结果:ATRA诱导后多巴胺能神经元特异性酪氨酸羟化酶(TH)染色呈阳性,细胞有多个细长突起,与对照组比较,诱导7 d后多巴胺神经元特异性TH染色阳性细胞率均明显升高(P〈0.01);各诱导组RA受体βmRNA的表达量均明显升高(P〈0.01),而且存在时间依赖性。结论:ATRA能显著提高NSCs分化为多巴胺能神经元的比例,同时发现细胞维甲酸A受体(RAR)βmRNA表达增加,RARβ激活可能促进NSCs向多巴胺神经元方向的分化。

关 键 词:全反式维甲酸  细胞分化  神经干细胞  多巴胺神经元  大鼠

Differentiation of midbrain-derived neural stem cells into dopamine neurons induced by alltrans retinoicacid
SHENG Hansong,ZHANG Nu,LIN Fengchun,ZHOU Hui,LIN Jian,YIN Bo. Differentiation of midbrain-derived neural stem cells into dopamine neurons induced by alltrans retinoicacid[J]. Journal of Wenzhou Medical College, 2011, 41(1): 6-9,12
Authors:SHENG Hansong  ZHANG Nu  LIN Fengchun  ZHOU Hui  LIN Jian  YIN Bo
Affiliation:SHENG Hansong,ZHANG Nu,LIN Fengchun,ZHOU hui,LIN Jian,YIN Bo.De-partment of Neurosurgery,the Second Affiliated Hospital of Wenzhou Medical College,Wenzhou,325027
Abstract:Objective: To observe the role of all-trans retinoic acid(ATRA) in inducing differentiation of rat midbrain-derived neural stem cells(NSCs) and its possible molecclar mechanism.Methods: Pregnant 14-15 day rats midbrain-derived NSCs were obtained by mechanical isolation and serum-free culture.Neural stem cells were induced by 1μmol/L ATRA on day 1,day 3,day 5 and day 7.The roles of ATRA in inducing the differentiation of NSCs were observed with immunofluorescent staining,and the expression of retinoic acid receptor(RAR) gene was determined with semi-quantitative RT-PCR.Results: After ATRA induction,neural stem cells were positive for tyrosine hydroxylase(TH),the rate of TH-positive cells significantly increased in the ATRA 7-day groups(P <0.01).Compared with the control group,RARβmRNA expression significantly increased in each group(P <0.01).That ATRA could induce the expression of RARβmRNA was a time dependent manner.Conclusion: ATRA can greatly increase the percentage of neurons differentiation from rat embry-onic neural stem cells and up-regulate the expression of RARβmRNA in rat midbrain-derived NSCs.Activation of RARβ may promote the differentiation of NSCs into dopaminergic neurons.
Keywords:all-trans retinoic acid  cell differentiation  neural stem cells  dopaminergic neurons  rats  
本文献已被 CNKI 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号