ALT测定中两种波长设置对不同浓度溶血影响程度的控制

目的观测不同波长设置时，3种溶血程度对丙氨酸氨基转移酶（ALT）测定的影响。方法用Sysmex XT-1800i全自动血细胞分析仪测定血清中血红蛋白（Hb）浓度。应用Olympus AU2700全自动生化分析仪在波长设置于340nm和340／660nm时检测20例住院患者，在不溶血和溶血程度在Hb为2．0、5．0、8．0g／L时ALT指标的变化。结果应用配对设计差值均数t检验得出，波长340nm时，与无溶血比较，Hb-2．0g／L时差异无统计学意义（P〉O．05）；Hb=5．0g／L时显著增高，差异有统计学意义（P〈O．05）；Hb-8．0g／L时显著增高，差异有统计学意义（P〈0．01）。波长340／660nm时，与无溶血比较，Hb-2．0g／L时差异无统计学意义（P〉0．05）；Hb-5．0g／L时差异无统计学意义（P〉0．05）；Hb-8．0g／L时显著增高，差异有统计学意义（P〈0．01）。无溶血标本在两种波长设置检测结果差异无统计学意义（P〉0．05）。结论一定的溶血会影响ALT检测，不同波长设置抗干扰能力不同，340／660nm优选。

Control of the influence of detecting wavelengths on the different degrees of hemolysis in ALT determination

Objective To observe the effects of three degrees of hemolysis in ALT determination with two different wavelengths. Methods To determine the concentration of hemoglobin in serum with Sysmex XT-1800i automatic blood analyzer, and determine the activities of ALT in serums, in which the concentration of hemoglobin has been determined（2.0 g/L,5.0 g/L,8.0 g/L） with Olympus AU2700（340 nm,340/660 nm）. The serum were collected from 20 patients in the hospital. Results By means of partnership difference t-test ,when single wavelength is at 340 nm, there is no remarkable difference（P〉0.05） between serum without hemolysis and 2.0 g/L concentration in Hb ;but there is remarkable difference（P〈0.05） between serum without hemolysis and 5.0 g/L concentration in Hb ; There is remarkable difference（P〈0.01） between serum without hemolysis and 8.0 g/L concentration in Hb. By using double wavelengths（340/660 nm）, there is no remarkable difference（P〉0.05） between serum without hemolysis and 2.0 g/L concentration in Hb ; There is no remarkable difference（P〉0.05） between serum without hemolysis and 5.0 g/L concentration in Hb ; There is remarkable difference（P〈0.01） between serum without hemolysis and 8.0 g/L concentration in Hb. There is no remarkable difference（P〉0.05） between single wavelength （340 nm） and double wavelengths（340/660 nm）if the serum is normal without hemolysis. Conclusion Control of influence on different degrees of hemolysis in ALT determination between single wavelength （340 nm） and double wavelengths （340/660 nm） is different. With single wavelength（340 nm）, the exact results of ALT activity can be made if Hb interference is low. Proper wavelengths （340/660 nm） should be selected according to the increasing Hb interferences.