An immunohistochemical study of the breast using antibodies to basal and luminal keratins,alpha-smooth muscle actin,vimentin, collagen IV and laminin |
| |
Authors: | Werner Böcker Bert Bier Götz Freytag Bettina Brömmelkamp Ernst-Dieter Jarasch Georg Edel Barbara Dockhorn-Dworniczak Kurt W. Schmid |
| |
Affiliation: | (1) Gerhard Domagk Institute of Pathology, University of Münster, Domagkstr. 17, W-4400 Münster, Federal Republic of Germany;(2) Institute of Cell and Tumour Biology, German Cancer Research Centre, University of Heidelberg, Im Neuenheimer Feld 280, W-6900 Heidelberg, Federal Republic of Germany |
| |
Abstract: | Summary The distribution of simple epithelial (K8/18/ 19) and basal (myoepithelial) (K5/14) keratins, -smooth-muscle actin, vimentin, collagen IV and laminin in normal mammary glands and in benign proliferative lesions was studied using monoclonal antibodies (mAbs). These antibodies (Abs) identified myoepithelial cells and luminal cells specifically. In lesions with adenosis and papillomas, the two-layered formation resembled that of normal glands with a purely myoepithelial-epithelial differentiation. In scleradenotic lesions, the main cell was of myoepithelial immunophenotype with intermixed trabecular-tubular proliferations of simple-type epithelium. The sclerosis seems to be the result of an irregular basal lamina synthesis by the myoepithelial cells. In contrast to these lesions, epitheliosis represents a purely intraluminal cell proliferation of clearly simple epithelial immunophenotype and of cells with a basal keratin phenotype, lacking myoepithelial differentiation antigen actin. The basal keratin type epithelium may represent post-stem or intermediate cells developing into luminal epithelium. Epitheliosis appears to be a purely epithelial hyperplasia with striking similarity to the regeneration of normal breast epithelium. The different proliferative patterns may give an explanation for differences in potential cancer risks of patients with these lesions.Dedicated to Prof. Dr. G. Seifert on the occasion of his 70th birthday |
| |
Keywords: | Hyperplastic breast lesions Anti-keratin antibody Anti-smooth muscle actin antibody Anti-vimentin antibody Anti-collagen IV antibody Immunohistology |
本文献已被 SpringerLink 等数据库收录! |
|