菟丝子提取物对MPP+诱导的PC12细胞凋亡的保护作用

目的:建立1-甲基-4-苯基吡啶(1-m ethyl-4-phenylpyrid in ium ion,MPP )诱导的大鼠肾上腺髓质嗜铬细胞瘤细胞(Pheochromocytom a,PC12)凋亡模型,探讨菟丝子提取物对MPP 诱导的PC12细胞凋亡的保护作用。方法:用四唑盐(MTT)方法检测细胞活力,用Hoechst 33258染色法观察细胞形态变化,用流式细胞技术检测细胞凋亡比例。结果:浓度为0.15 mM MPP 作用于PC12细胞48 h诱导其产生典型的凋亡特征,50 mg/L菟丝子提取物具有抗凋亡作用。结论:菟丝子提取物对MPP 诱导的PC12细胞凋亡有明显的保护作用。

Protection of Semen Cuscuta extracts from apoptosis PC12 cell of induced by 1-methyl -4 -phenyipyridinium

AIM: To establish an apoptotic model induced by 1-methyl-4-phenylpyridinium ion(MPP~ ) in PC12 cell and assess the effect of Semen Cuscuta extracts on protecting PC12 cell from apoptosis induced by MPP~ . METHODS: The cell viability was analyzed by MTT method,the morphological changes were observed by Hoechst 33258 staining assay,and the apoptosis rate was measured by flow cytometry analysis(FCM).(RESULTS:) Treatment of 0.15 mM MPP~ for 48 h induced apoptosis in PC12 cells;concentration of 50 mg/L Semen Cuscuta extracts could protect cells from apoptosis induced by MPP~ . CONCLUSION: MPP~ -induced apoptosis in PC12 cell is greatly inhibited by Semen Cuscuta extracts.