转化生长因子β1和同种异体软骨细胞及高孔隙率聚乳酸复合物修复兔耳廓软骨缺损

目的 观察加入转化生长因子-β1(transforming growth factor-beta1，TGF-β1)的同种异体软骨细胞／高孔隙率聚乳酸(poly-DL-lactide，PDLLA)支架复合物修复兔耳廓软骨缺损的效果。方法 18只大白兔随机分为同种异体软骨细胞／PDLLA复合物加TGF-β1组(复合物组)、同种异体软骨细胞／PDLLA复合物组(复合物对照组)和不用任何修复材料的空白对照组，每组6只。分别于4、12、18周取材，行HE和甲苯胺蓝染色，观察各组兔耳廓软骨缺损修复情况。结果 术后18周，TGF-β1复合物组修复软骨缺损的效果无论从大体标本观察或是病理组织学检查都比复合物对照组的修复效果好，空白对照组为纤维组织修复。结论 同种异体软骨细胞／PDLLA复合物移植可形成组织工程化软骨修复兔耳廓软骨缺损，TGF-β1可提高同种异体软骨细胞／PDLLA复合物移植修复兔耳廓软骨缺损的质量。

Repair of the rabbit ear cartilage defects with transforming growth factor-beta1 and allogenic chondrocyte/poly-DL-lactide higher porosity polymer]

OBJECTIVE: To study the repair of the rabbit ear cartilage defects with transforming growth factor-beta1 (TGF-beta1) and allogenic chondrocyte/poly-DL-lactide (PDLLA) higher porosity polymer. METHODS: A total of 18 rabbits were divided into the TGF-beta1 and chondrocytes/PDLLA graft group, the chondrocytes/PDLLA graft group, and the blank control group. Samples were taken out at 4, 12 and 18 weeks after implantation. The histological characteristics were investigated. RESULTS: After 18 weeks of the transplantation, the repaired effects of TGF-beta1 and allogenic chondrocytes/PDLLA graft group was better than the allogenic chondrocytes/PDLLA graft group not only on general sample but also histology, and the blank control group were repaired with fibrous tissue. CONCLUSIONS: The allogenic chondrocytes/PDLLA graft could repair the rabbit ear cartilage defects while TGF-beta1 could improve the quality of the rabbit ear cartilage defects.