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Adrenal release of catecholamines and Met-enkephalin before and after stress as measured by a novel in vivo dialysis method in the rat |
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| Authors: | H. Jarry, E.-M. Dü ker,W. Wuttke, |
| Affiliation: | 1 Deutsches Primatenzentrum, Abt, für Reproduktionsbiologie, Kellnerweg 4, D-3400, Göttingen, F.R.G. 2 Georg-August-Universität, Frauenklinik, Abt. für klin, und exp. Endokrinologie, Humboldtallee 19, D-3400, Göttingen, F.R.G. |
| Abstract: | A dialysis tubing was implanted in the adrenal gland of rats. Adrenomedullary secretion products were dialysed into Ringer solution in the awake animal. Catecholamines (CAs) and methionine-enkephalin (Met-Enk) were measurable under resting conditions. Epinephrine, norepinephrine and Met-Enk-like immunoreactive material increased following application of an immobilization stress for 5 min. The results demonstrate that adrenomedullary CAs and peptides are coreleased. They also demonstrate that Met-Enk-like immunoreactive material is released under stress. |
| Keywords: | rat adrenal medulla stress dialysis catecholamine methionine-enkephalin |
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