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应用差异显示从高低转移癌系克隆出基因G3BP
引用本文:刘宇欣,郑杰,方伟岗. 应用差异显示从高低转移癌系克隆出基因G3BP[J]. 肿瘤, 1999, 19(5): 267-271
作者姓名:刘宇欣  郑杰  方伟岗
作者单位:北京医科大学病理学系!100083
摘    要:目的 以 m R N A 差异显示技术对比研究具有不同转移潜能的癌系,克隆与肿瘤转移相关的基因。方法 以肺巨细胞癌细胞系 P G 具有不同转移潜能的亚系( B E1, L H7)为研究对象,应用 m R N A 差异显示技术克隆差异片段,经 Northern 杂交验证后测序,进入 Gen Bank 数据库检验同源性。结果 m R N A 差异显示技术对比高低转移癌系m R N A,分别建立了高低转移相关基因资源库。其中一个849 bp 片段在高转移亚系( B E1)低表达,而在不转移亚系( L H7)高表达,两者差异显著,经 Gen Bank Blastn 检索,与 G3 B P( Ras G T Pase Activating protein S H3 dom ain bindingprotein)同源性高达 99% 。结论 在高低转移癌系中 G3 B P的表达具有显著差异,提示 G3 B P与肿瘤的转移表型有一定相关性,为 G3 B P功能的研究提供了新的线索。

关 键 词:肺肿瘤  肿瘤转移  基因  G3BP  肿瘤细胞  培养的
修稿时间:1999-03-17

IDENTIFICATION OF G3BP DIFFERENTIALLY EXPRESSED IN HUMAN LUNG GIANT CELL CARCINOMA SUBLINES WITH DIFFERENT METASTATIC CAPACITY BY MESSENGER RNA DIFFERENTIAL DISPLAY
LIU Yu-xin,ZHENG Jie,FANGWei-gang,et al.. IDENTIFICATION OF G3BP DIFFERENTIALLY EXPRESSED IN HUMAN LUNG GIANT CELL CARCINOMA SUBLINES WITH DIFFERENT METASTATIC CAPACITY BY MESSENGER RNA DIFFERENTIAL DISPLAY[J]. Tumor, 1999, 19(5): 267-271
Authors:LIU Yu-xin  ZHENG Jie  FANGWei-gang  et al.
Abstract:Objective The Messenger RNA Differential Display technique was used to identify mRNAs expressed differentially in human lung giant cell carcinoma sublines with different metastatic capacity and to find out the metastasis related gene.Methods Different metastatic potency sublines of human lung giant cell carcinoma PG line was studied by mRNA differential display technique.After Northern hybridization and sequencing,the DNA sequence was checked with GenBank data for homology.Results A clone was isolated,which is uniquely expressed in the low metastatic subline LH7.DNA sequence of the clone was checked in the database of Genbank by Blastn and indicated a significant homology to G3BP(Ras GTPase Activating protein SH3 domain binding protein).Conclusions G3BP is uniquely expressed in the low metastatic human lung giant cell carcinoma subline LH7,so it might have some relationship with cancer metastasis.$$$$
Keywords:Lung neoplasms  Neoplasm metastasis  Gene  G3BP  Tumor cells  cultured
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