耐甲氧西林金黄色葡萄球菌对不同消毒剂的抗性研究

目的了解耐甲氧西林金黄色葡萄球菌（MRSA）对不同消毒剂的抗性，探讨合理的消毒剂使用方案。方法以头孢西丁纸片扩散法（K—B法）筛选MRSA菌株，检测70％乙醇、0．5％氯己定醇、10％碘伏、0．5％氯已定对MRSA的最小抑菌浓度（MIC），比较MIC差异，并进行定量的杀菌试验检测。结果氯已定、碘伏、氯已定醇、乙醇的MIC50分别为64．0、64．0、2．4、1．0~g／mL；在30s内，乙醇对MRSA的杀菌率在90％以上，在2min内，乙醇可清除MRSA。结论MRSA对氯已定、碘伏有抗性，对氯已定醇和乙醇敏感。

Resistance of different disinfectants on methicillin resistant staphylococcus aureus

Objective To investigate the degree of resistance of different disinfectants on methicillin--resist- ant Staphylococcus aureus （MRSA）, in order to explore the rational use of disinfectants. Methods Cefoxitin disk diffusion （KB method） was applied to screen MRSA strains; the minimum inhibitory concentration （MIC） of 70% ethanol, 0.5 % chlorhexidine alcohol, 10% povidone-iodine, and 0.5 chlorhexidine against MRSA were tested; the MIC differences were compared; quantitative germicidal test of disinfectants were conducted. Results The MICs0 of chlorhexidine, povidone-iodine, chlorhexidine alcohol and ethanol were 64.0 μg/mL, 64.0 μg/mL, 2.4 μg/mL and 1.0 μg/mL, respectively. In 30 seconds, the bactericidal rate of ethanol against MRSA was 90%. In 2 minutes, the rate was 100%. Oonclusion MRSA is resistant to chlorhexidine and povidone-iodine while sensitive to chlorhexidine and ethanol,