卵巢癌组织中STAT3和免疫效应细胞的表达及其相关性分析

目的:研究卵巢上皮癌细胞中STAT3表达与DC、CD4+、CD8+表达之间的关系及临床意义,探讨卵巢癌发生过程中肿瘤免疫逃逸的机制。方法:选择42例卵巢上皮性癌患者为研究对象,25例卵巢良性肿瘤患者及13例正常卵巢组织为对照。用免疫组织化学SABC法检测组织中STAT3与DC、CD4+、CD8+的表达,并进行半定量及相关性分析。结果:(1)STAT3在卵巢上皮性癌中表达增高,与良性和正常对照组的差异有统计学意义(P<0.005);按FIGO分期,Ⅰ~Ⅱ期与Ⅲ~Ⅳ期的差异有统计学意义(P<0.05);(2)DC、CD4+在卵巢上皮性癌中表达降低,与良性和正常对照组的差异有统计学意义(P<0.005);按FIGO分期,Ⅰ~Ⅱ期与Ⅲ~Ⅳ期的差异有统计学意义(P<0.05);(3)CD8+在卵巢上皮性癌中表达降低,与良性和正常对照组的差异有统计学意义(P<0.005);按FIGO分期,Ⅰ~Ⅱ期与Ⅲ~Ⅳ期比较,低表达率增高,但无统计学意义(P>0.05);(4)卵巢上皮癌组织中STAT3表达与DC、CD4+、CD8+表达之间呈负相关,相关系数分别为-0.230、-0.081、-0.102,P均<0.05。结论:STAT3与DC、CD4+、CD8+的表达可作为判断卵巢癌恶性程度的指标;STAT3与DC、CD4+、CD8+在肿瘤组织的表达呈负相关。各因素间相互影响,可能是导致肿瘤逃脱机体免疫监视的机制之一。

Expression and relativity of STAT3 and immunity effect cell in epithelial ovarian carcinoma

Objective：To study the expression of STAT3,DC,CD4＋,CD8＋ in ovarian epithelial carcinoma and explore the relationship between them and the clinical significance,to discuss a mechanism of the tumor escaping immunity surveillance in the ovary occurrence process.Methods：Tissue samples from 42 women with primary ovarian epithelial carcinoma（OVCA）,25 with benign ovarian tumor（OVBT） and 13 with normal ovarian tissue（NOV） were collected.SABC immunohistochemical staining was used to determine the expression of STAT3 and DC,CD4＋,CD8＋,also carry on half fixed amount and relativity analysis.Results：（1）Semi-quantitative scoring showed that STAT3 expression in OVCA was significantly higher than that in OVBT and NOV（P0.005）.In OVCA,tumor tissue expressed STAT3 at significantly higher levels in Ⅲ～Ⅳ than Ⅰ～Ⅱ by the FIGO staging（P0.05）.（2）DC,CD4＋ was lower expressed significantly in OVCA than that in OVBT and NOV（P0.005）.Tumor tissue expressed DC,CD4＋ at significantly lower levels in Ⅲ～Ⅳ than Ⅰ～Ⅱ by the FIGO staging（P0.05）.（3）CD8＋was also lower expressed significantly in OVCA than that in OVBT and NOV（P0.005）.The Ⅲ～Ⅳ and Ⅰ～Ⅱ comparison by the FIGO staging,the low expression rate increased,but had no significant differences（P0.05）.（4）The level of STAT3 expression was negatively related with level of DC,CD4＋ and CD8＋（P0.05）.Conclusion：The expressions of STAT3 and DC,CD4＋,CD8＋may play an important role in the development of ovarian tumors.The ovarian epithelial carcinomas with STAT3 higher expression and those with DC,CD4＋,CD8＋ lower expression might have worse prognosis.STAT3 inhibited the function of DC,CD4＋,CD8＋T cell,which might be one of the mechanisms of tumor evasion of immune surveillance.