| 烧伤血清和痂下水肿液对中性粒细胞凋亡的影响 |
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| 引用本文： | 李志清,杨宗城,黄跃生,王甲汉,周一平.烧伤血清和痂下水肿液对中性粒细胞凋亡的影响[J].解放军医学杂志,2001,26(5):342-343. |
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| 作者姓名： | 李志清 杨宗城 黄跃生 王甲汉 周一平 |
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| 作者单位： | 1. 广州第一军医大学南方医院
2. 第三军医大学烧伤研究所 |
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| 摘 要： | 为了解严重烧伤后中性粒细胞（PMN）凋亡情况及烧伤血清和痂下水肿液（STF）对中性粒细胞凋亡的影响。将24只健康大鼠随机分为烫伤组（30%Ⅲ度烫伤）和对照组，分离伤后0、12、24hPMN，继续培养16h，碘化丙啶（propdiium oddide,PI)染色，流式细胞仪检测凋亡百分率，并用烧伤血清和痂下水肿液分别刺激对照组大鼠PMN，检测凋亡百分率，结果发现：烫伤组大鼠PMN凋亡百分率较对照组大鼠降低，烧伤血清和痂下水肿液均抑制PMN凋亡并呈剂量依赖关系。说明严重烧伤后PMN凋亡延迟，烧伤血清和痂下水肿液在其中可能起重要作用。
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| 关 键 词： | 烧伤 细胞凋亡 血清 痂下水肿瘤 中性粒细胞 |
| 修稿时间： | 2000年5月23日 |
EFFECTS OF BURN SERA AND SUBESCHAR TISSUE FLUID ON APOPTOSIS OF POLYMORPHONUCLEAR LEUKOCYTES IN VITRO |
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| Li Zhiqing,Yang Zongcheng,Huang Yuesheng et al.EFFECTS OF BURN SERA AND SUBESCHAR TISSUE FLUID ON APOPTOSIS OF POLYMORPHONUCLEAR LEUKOCYTES IN VITRO[J].Medical Journal of Chinese People's Liberation Army,2001,26(5):342-343. |
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| Authors: | Li Zhiqing Yang Zongcheng Huang Yuesheng |
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| Institution: | Li Zhiqing,Yang Zongcheng,Huang Yuesheng et al . Nanfang Hospital,First Military Medical University,Gangzhong 510515 |
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| Abstract: | To elucidate apoptosis of polymorphonuclear leukocytes (PMN) in early postburn stage and the effects of burn sera and subeschar tissue fluid (STF) on apoptosis of PMNs, 24 Wistar rats were divided into scald group and control group (no scald group) randomly. PMNs were harvested at 0, 12, 24h after scald injury,and percentage of apoptotic PMNs was measured by flow cytometry.Apoptosis of PMNs was again assayed with flow cytometer after being incubated with burn sera or STF harvested from scalded rats. The result showed that the percentage of apoptotic PMNs in scalded group were lower than that in control group,indicating burn sera and STF could inhibit apoptosis of PMNs in a dose dependent manner. It suggested that spontaneous apoptosis of PMNs is inhibited in early postburn stage, and burn sera and STF play an important role in this process. |
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| Keywords: | burns leukocytes apoptosis |
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