| 尿毒清颗粒中黄芪甲苷的含量测定 |
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| 引用本文： | 邹剑成,周洪波.尿毒清颗粒中黄芪甲苷的含量测定[J].中国药业,2009,18(9):27-28. |
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| 作者姓名： | 邹剑成 周洪波 |
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| 作者单位： | 1. 广东省中山市博爱医院,广东,中山,528403
2. 广东药学院中山校区,广东,中山,528405 |
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| 摘 要： | 目的建立尿毒清颗粒中黄芪甲苷的含量测定方法。方法采用高效液相色谱-蒸发光龊射检测器（HPLC—ELSD）法，色谱柱为Thermo Hypersil ODS-2柱（150mm×4．6mm，5μm），流动相为乙腈-水（30：70），柱温为30℃，蒸发光散射检测器漂移管温度105℃，载气流量2．8L／min。结果黄芪甲苷进样量在0．6276～3．9225μg范围内与峰面积呈良好的线性关系（r=0．9993），平均回收率为97．45％．RSD为3．44％（n=6）。结论HPLC—ELSD法准确、重现性好，可作为尿毒清颗粒的质量控制方法。
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| 关 键 词： | 高效液相色谱-蒸发光散射检测器法 黄芪甲苷 尿毒清颗粒 |
Determination of Astragaloside in Niaoduqing Granules by HPLC-ELSD |
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| Zou Jiancheng,Zhou Hongbo.Determination of Astragaloside in Niaoduqing Granules by HPLC-ELSD[J].China Pharmaceuticals,2009,18(9):27-28. |
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| Authors: | Zou Jiancheng Zhou Hongbo |
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| Institution: | 1. Zhongshan Boai Hospital, Zhongshan, Guangdong, China 528403; 2. Zhongshan Experimental Center, Guangdong College of Pharmacy, Zhongshan, Gnangdong, China 528405) |
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| Abstract: | Objective To establish a HPLC- ELSD determination method for astragaloside in Niaoduqing Granules. Methods The separation was performed on Thermo ODS- 2 Hypersil column（150 mm ×4.6 mm, 5 μm）, the mobile phase was acetonitrile- water（30 ： 70）, the flow rate was 1 mL/min, the column temperature was 30 ℃. The evaporative light scattering detector（ELSD） as the detector, the temperature of the drift tube was set at 105 ℃, and the flow of gas was set at 2. 8 L/min. Results The linear range of astragaloside was 0. 627 6-3. 922 5 μg （r=0. 999 3）. The recovery rate was 97.45%, RSD was 3.44% （n =6）. Conclusion The method is accurate, precise, repeatable and suitable for the determination of astragaloside in Niaoduqing Granules. |
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| Keywords: | HPLC - ELSD astragaloside Niaoduqing Granules |
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