大剂量维生素C治疗大鼠急性脊髓损伤初步研究

目的　研究大剂量 Vitam in C对大鼠急性脊髓损伤 (SCI)疗效并与甲基强的松龙 (MP)相比较。方法SD大鼠 4 8只随机分为 A组 (SCI+MP) ,B组 (SCI+Vitam in C) ,C组〔 SCI+生理盐水 (NS)〕,Allen's模型 (7g× 4cm )致大鼠 T1 1 急性 SCI;伤后 0 .5 h A组、B组分别腹腔注射 MP 30 m g/ kg、Vitam in C 2 0 0 mg/ kg,C组腹腔注射等量 NS。观察伤后 4 8h脊髓 HE染色 +尼氏染色、含水量及伤后 2周斜板实验 +Gale评分。结果　 A、B组伤后 4 8h脊髓出血坏死程度及含水量明显轻于创伤组 ,神经元残存 Olsiwski氏小体数量及伤后 2周斜板实验 +Gale评分也明显高于 C组 (P<0 .0 5 ) ,而 A、B组间差异无显著性 (P>0 .0 5 )。结论　大剂量 Vitamin C早期使用对大鼠急性脊髓损伤有治疗作用 ,但效果不优于大剂量 MP。

Preliminary Study of the Effects of High-dose Vitamin C on Acute Spinal Cord Injury in Rats

OBJECTIVE: To investigate the therapeutic effect of high-dose Vitamin C on acute spinal cord injury (SCI) in rats. METHODS: Forty-eight SD rats were randomly divided into 3 groups (A, B, C), and SCI was made by Allen's Mode (7 g x 4 cm) on spinal cord T11 extradurally. Half an hour after SCI, the rats of groups A and B were given methylprednosolone (30 mg/kg, i.p.) and Vitamin C (200 mg/kg, i.p.) respectively. The rats of Group C recevied normal saline i.p. only. The results of hematoxylin staining+Olsiwski corpuscle staining and the moisture of spinal cord 48 h after SCI were observed. Inclined plate and Gale scale were observed 2 weeks after SCI. RESULTS: In both group A and group B, there was less scope of neuro-necrosis and bleeding, less degree of hydrops and more Olsiwski corpuscle count in neure of injured areas 48 h after injury, and much better behavioral assessment (inclined plate+Gale scale) 2 weeks after injury in comparison with those in group C (P<0.05), while there were no diffferences between group A and group B statistically (P>0.05). CONCLUSION: Early administration of high-dose Vitamin C is effective for treating SCI, but its effectiveness is not superior to that of high-dose methylprednisolone.