脾经穴位注射香菇多糖对脾气虚模型家兔免疫功能的影响

目的:证实穴位给药的药效反应是否具有特异性和循经性以及脾经穴位与机体的免疫功能相关。方法:健康新西兰家兔54只,随机分为正常组、模型组、肌肉治疗组、三阴交治疗组、地机治疗组、血海治疗组。每日以100%生大黄水煎剂灌胃(15mL/kg),连续10d,造成家兔脾气虚模型。自造模第2天起,各治疗组按不同给药途径隔日注射香菇多糖(LNT,0.025mg/kg),共5次。实验结束,各组按郭峰法测红细胞免疫功能(RBC-C3bR、RBC-IC),免疫比浊法测血清IgM浓度,同时观察家兔体征变化。结果:不同途径注射LNT均能不同程度地纠正脾气虚家兔RBC-C3bR花环形成率及血清IgM浓度的下降,以三阴交治疗组和血海治疗组效果最好(与模型组相比,P0.01或P0.05)。结论:脾经不同穴位点注射LNT改善脾气虚模型家兔的体征及免疫功能的作用比肌肉注射途径更为显著,经络穴位给药的药效具有特异性,经脉脏腑功能相关。

Effects of acupoint-injection of Lentinan on the immunologic funtion in rabbits with spleen-qi deficiency

To observe the effect of Spleen-Meridian-acupoint injection of Lentinan on the immunologic function in spleen-deficiency rabbits. Methods A total of 54 Newzealand rabbits were randomly divided into normal (n=10),model (n=8),intramuscular injection (n=10),Sanyinjiao (SP 6,n=10),Diji (SP 8,n=8) and Xuehai (SP 12,n=8) groups. Spleen-qi deficiency model was established by intragastric administration of 100% crude Radix et Rhizoma Rhei decoction (15 mL/kg/day× 10 d ),and then Lentinan (LNT,0.025 mg/kg/2 day×5) was injected into the aforementioned acupoints of the Spleen Meridian. The erythrocyte immunologic function(RBC-C 3 bR,RBC-IC),hemolysin(IgM)and changes of physical signs of the rabbits were observed. Results In comparison with the control group,the rabbits' body weight,rectal temperature,RBC-C 3 bR% and serum IgM level were decreased significantly in model group ( P<0.05 ); while in comparison with the model group,the body weight in SP 8 group,retal temperature in SP 9 and SP 8 groups,RBC-C 3 bR% in SP 9 and SP 12 groups,and serum IgM levels in SP 9,SP 8 and SP 12 groups increased considerably ( P<0.05 ,P<0.01 ). Comparison among the 4 treatment groups showed that the effect of SP 12 was superior to that of intramuscular injection group in upregulaing RBC-C 3 bR%,and the effects of SP 9,SP 8 and SP 12 groups were significantly superior to those of intramuscular injection group in upregulating serum IgM level ( P<0.05 ). Conclusion The Spleen-Meridian-acupoint injection of LNT is superior to that of intramuscular injection of LNT in improving the spleen-qi deficiency rabbits' symptoms and immunologic function.