血管内皮生长因子在高原脑水肿形成中作用的实验研究

目的:探讨血管内皮生长因子(VEGF)在高原脑水肿形成中的作用。方法:建立大鼠模拟高原模型,应用脑干湿重比率法定量脑水肿情况、应用荧光素钠透过率测定BBB通透性、应用实时荧光定量RT-PCR法检测脑组织VEGF mRNA含量以及应用蛋白印迹法半定量脑组织VEGF含量。结果:大鼠在高原24 h后脑组织含水率明显增高(P<0.05),荧光素钠透过率显著增加(P<0.01);VEGF mRNA转录及其表达显著增高(P<0.001)。结论:VEGF表达在高原脑水肿形成中起重要作用。

An Experimental Study on the Role of Vascular Endothelial Growth Factor in the Formation of High Altitude Cerebral Edema

Aim:To investigate the role of vascular endothelial growth factor in the formation of high altitude cerebral edema.Methods:Wistar rats were exposed to low-pressure cabin for 24 hours and mimiced high altitude of 7 000 meters.The ratio of dry and wet brain weight was calculated and the sodium fluorescein leakage was calculated to evaluate the cerebral edema and the blood brain barrier permeability.Also the brain tissue was processed for Western blots to detect VEGF expression and for realtime quantitative RT-PCR to detect expression of VEGF mRNA.Results:After 24 hours of rats exposed in mimiced the high altitude of 7 000 meters,The ratio of water content of brain tissue increased significantly(P<0.05),the permeability coefficients of sodium fluorescein increased significantly,too(P<0.01).Meanwhile,it was observed that the expression of VEGF-mRNA and VEGF increased more significantly(both P<0.001).Conclusion:It is suggested that the expression of VEGF involves in the damage of blood brain barrier and may play an important role in the formation of high altitude cerebral ischemia.