肾母细胞瘤中PTEN、MMP-7的检测

目的:检测肾母细胞瘤(nephrohlastoma,wilms tumor,WT)组织中PTEN和MMP_7中的表达。方法:用免疫组化方法检测12例瘤旁正常肾组织和42例WT组织中PTEN、MMP_7的表达。结果:PTEN在WT组织中的表达明显低于正常瘤旁肾组织(P<0.05)。PTEN的表达与性别、年龄和病理学分型无关(P>0.05),而与临床分期明显相关(P<0.05)。12例对照组织标本中MMP_7未见表达,42例WT组织标本中MMP_7在胞浆表达率54.21%。MMP_7在预后良好(favorable histological types,FH)组中的阳性表达率明显低于在预后不良(unfavorable histological types,UH)组中的阳性表达率(P<0.05);PTEN和MMP_7呈负相关。结论:联合检测WT组织中PTEN和MMP_7的表达,能更好评估WT的侵袭和转移等生物学行为,两者可作为判断WT侵袭性的标志物。

Detecting of PTEN and MMP-7 in nephroblastoma

Objective: To detect the expression of PTEN and MMP-7 in nephroblastoma.Methods: With immunohistochemistry to detect the expression of PTEN and MMP-7 in 12 cases of normal renal tissues near the tumor and 42 cases of children nephroblastoma tissues.Results: Expression of PTEN was lower in nephroblastoma than that in normal renal tissues(P<0.05).Expression of PTEN has no significant relativity with sex,age and pathologic types(P>0.05).But significant relativity was found between PTEN and clinical stage(P<0.05).There is no MMP-7 positive expression in 12 cases normal renal tissues,and 54.21 % positive rate in 42 cases nephroblastoma tissues.There is a significant higher expression of MMP-7 in unfavorable histologic types nephroblastoma group than that of in favorable histologic types nephroblastoma group.A negative relativity was found between PTEN and MMP-7 in nephroblastoma tissues.Conclusion: United detecting of PTEN and MMP-7 in nephroblastoma tissues,maybe helpful to estimate the biological behavior of nephroblastoma,and they can be the signal substance of estimating nephroblastoma's metastasis.