补阳还五汤对小鼠脑缺血后NOS活性的影响

目的：观察补阳还五汤对小鼠局灶性脑缺血后脑组织内NOS活性的影响。方法：局灶性脑缺血模型由丝线结扎一侧带迷走神经的颈总动脉制成，用NOS试剂盒和紫外分光光度测定NOS活性。结果：缺血后脑组织内NOS显升高，以缺血4h达高峰．补阳还五汤组NOS活性比缺血对照组显降低，以缺血4h最明显。结论：补阳还五汤能抑制缺血后脑组织内NOS活性的升高，对缺血后脑组织具有保护作用。

Effect of Buyang Huanwu Decoction (补阳还五汤) on cerebral nitric oxide synthase level during focal cerebral ischemia in mice

Objective: The purpose of this study was to observe the effect of Buyang Huanwu Decoction (BYHWD) on cerebral nitric oxide synthase level during focal cerebral ischemia in mice. Methods: Focal cerebral ischemia was produced by ligatad with a ligature of 1.0 suture material around left common carotid artery and its vagus nerve in urethane anestheticzed mice. The activity of brain nitric oxide synthase was measured by NOS reagent salver through ultraviolet spectroscopy. Results: The results showed that the activity of NOS in the cerebral tissue was increased in the ischemic hemisphere after induction of ischemia especially at 4 hours after the onset of ischemia. BYHWD group significantly decreased compared with cerebral ischemic group (P<0.05), especially at 4 hours after the onset of ischemia. Conclusion: BYHWD could significantly restrain the up regulated activity of NOS after focal cerebral ischemia to protect the cerebral ischemic lesion, especially at 4 hours after the onset of ischemia.