高脂饮食诱导C57BL/6J小鼠肥胖及减重手术模型的建立

 目的 建立饮食诱导的肥胖小鼠模型，研究建立肥胖小鼠胃袖状切除术模型的可行性及有效性。方法 将40只4周龄雄性SPF级C57BL/6J小鼠随机分为普通饮食组（n=20）和高脂饮食组（n=20），均喂养20周。20周后随机分为4个亚组：普通饮食假手术组，普通饮食手术组，高脂饮食假手术组，高脂饮食手术组。定期测量体重及空腹血糖，喂养20周后检测葡萄糖耐量、血脂水平及肝组织病理学。结果 高脂饮食喂养4周后，高脂饮食组小鼠体重即开始显著高于普通饮食小鼠（P < 0.05）。喂养20周后，与普通饮食小鼠相比，高脂饮食小鼠平均体重（42.71±2.30）g vs.（32.23±1.73）g]、血糖（8.82±0.51）mmol/L vs.（6.74±0.31）mmol/L]及血脂水平血清总胆固醇：（4.40±0.57）mmol/L vs.（2.31±0.35）mmol/L；血清三酰甘油：（2.26±0.25）mmol/L vs.（1.11±0.24）mmol/L]均明显升高（P均 < 0.01）。高脂饮食及普通饮食假手术组小鼠存活率为100%，高脂饮食手术组存活率为70%（7/10），普通饮食手术组存活率为80%（8/10）。术后第1周，各组小鼠体重均显著下降（P < 0.01）；术后第4周，高脂饮食及普通饮食手术组小鼠较术前体重下降约20%；术后第3周，高脂饮食及普通饮食假手术组小鼠体重恢复至术前水平。结论 高脂饮食喂养20周可诱导肥胖小鼠模型，进一步建立胃袖状切除术模型可行且有效。

Establishment of high-fat diet-induced obesity and sleeve gastrectomy in C57BL/6J mice

Objective To establish diet-induce obese model and explore the feasibility and effectiveness of sleeve gastrectomy (SG) model.Methods Forty 4-week-old male SPF C57BL/6J mice were randomly divided into two group: standard diet (SD) group (n=20), high-fat diet (HFD) group (n=20), and were fed for 20 weeks.After 20 weeks, they were randomly divided into 4 subgroups: SD+sham group, SD+SG group, HFD+sham group and HFD+SG group.Body weight and fasting blood glucose were measured, and glucose tolerance, blood lipid levels and histopathology were measured after 20 weeks.Results After 4 weeks, the average body weight in HFD group was significantly higher than those in SD group (P < 0.05).After 20 weeks, the average body weight (42.71±2.30) g vs. (32.23±1.73) g], blood glucose (8.82±0.51) mmol/L vs. (6.74±0.31) mmol/L] and the serum lipid concentrations total cholesterol: (4.40±0.57) mmol/L vs.(2.31±0.35) mmol/L; triacylglycerol: (2.26±0.25) mmol/L vs.(1.11±0.24) mmol/L] were significantly higher than those in SD group (all P < 0.01).The survival rate in HFD+sham group and SD+sham group were 100%, in HFD+SG group it was 70% (7/10) and in SD+SG group it was 80% (8/10).The body weight in each group decreased significantly in postoperative one week (P < 0.05);the mice lost about 20% of their preoperative weight in HFD+SG group and SD+SG group in the fourth week after surgery; the bodyweight recovered to the preoperative level in HFD+sham group and SD+sham group in the third week after surgery.Conclusion High-fat diet for 20 weeks could induce obese mice successfully, and sleeve gastrectomy model is effective and feasible.