Src激酶在正常发育和未成熟大鼠缺氧缺血脑白质损伤中的表达变化

目的 研究大鼠正常发育脑中和缺氧缺血性脑白质损伤中Src激酶活化的表达.方法 对出生后第3天SD大鼠幼仔进行右颈总动脉结扎,然后进行2.5 h缺氧(6％氧气)造模.通过苏木精-伊红(HE)染色、蛋白免疫印迹和免疫荧光染色观察神经病理学改变.收集出生后6、10、17天对照组和缺氧缺血(hypoxia-ischemia,H...

Expressions of Src kinases in normal developing brain and immature brains with hypoxic-ischemic white matter injury of rats

Objective To investigate the expressions of phosphorylated Src kinase in normal developing brains and immature brains with hypoxic-ischemic white matter injury of rats. Methods Three-day-old Sprague-Dawley rat pups were subjected to right carotid artery ligation,followed by 2.5 h of hypoxia (6% oxygen). The neuropathological changes were observed by hematoxylin-eosin (HE)staining,Western blot and immunofluorescence. Brain tissues were collected from control and hypoxia-ischemia (HI) group rats at postnatal day 6,10 and 17. Expression of phosphorylated Src was evaluated by Western blot and immunofluorescence.The relationship of phosphorylated Src kinases and oligodendrocytes (OLs) was evaluated by triple immunofluorescence staining. Results In the control group,the expression of phosphorylated Src kinases peaked at 6 and 10 days after birth.Phosphorylated Src kinases was predominantly present in mature OLs, which was rarely observed in oligodendrocyte precursor cells.In the 3-day rat hypoxic-ischemic white matter injury model,phosphorylated Src kinases decreased significantly 3 days after injury (6 days after birth) (P=0.0007,n=6-7 for each group).At 7 days after injury (10 days after birth) and 14 days (17 days after birth) after injury,phosphorylated Src kinases returned to the level of control group,and there was no statistically significant difference between control group and HI group (n=3 for each group). Conclusion In normal developing brains of rats,the expression of phosphorylated Src kinases has a time rhythm and decreases after HI injury.