替考拉宁高产菌选育及发酵条件优化

摘要：目的 获得一株稳定遗传的高产新菌株，并提高替考拉宁产量。方法 以替考拉宁产生菌游动放线菌T19为出发 菌株，通过常压室温等离子(ARTP)诱变技术，获得一株具有稳定遗传性的高产新菌株，并对转速、接种量、温度和pH等发酵 条件进行了优化，确定了最佳的发酵工艺条件，从而提高了替考拉宁的产量。结果 诱变后菌株的发酵效价水平较出发菌株提 高了42%；在转速为220 r/min，接种量为7.5%，温度为28℃，pH为6.5时，该菌株发酵效价水平比出发菌株提高了80%。结论 ARTP诱变技术可有效用于游动放线菌的诱变选育，可大幅度提高替考拉宁的产量，为其工业化生产奠定了基础。

Breeding of teicoplanin high-yield strain from actinomycetes and optimization of fermentation conditions

Abstract Objective To obtain a new strain with genetic stability and higher yield of teicoplain. Methods A new strain with stable heredity and high yield was obtained by the atmospheric pressure room temperature plasma (ARTP) mutagenesis treatment using a teicoplanin-producing bacteria Antinoplanes teichomycetics T19 as the starting strain. Fermentation conditions such as rotation speed, inoculation amount, temperature, and pH were optimized to improve the yield of teicoplanin. Results The fermentation titer of the mutagenized strain was 42% higher than that of the original strain. When rotation speed was 220 r/min, inoculation amount was 2%, temperature was 28℃, and pH was 6.5, the fermentation titer of this strain was 80% higher than that of the original strain. Conclusion The ARTP mutagenesis technology could be effectively used for the mutagenesis and breeding of the Actinoplanes to increase the yield of teicoplanin, which laid a foundation for its industrial