北极海洋来源真菌Aspergillus sp. HDN19-401次级代谢产物的研究

摘要：目的 研究北极海洋来源真菌Aspergillus sp. HDN19-401的次级代谢产物及其生物活性。方法 采用反相硅胶柱色谱、葡聚糖凝胶Sephadex LH-20、半制备HPLC等方法分离纯化该菌株的发酵粗提物，通过ESI-MS、NMR等波谱数据分析并与文献对比确定化合物的结构，最低抑菌浓度(minimal inhibitory concentration, MIC)采用微量肉汤稀释法测定，抗肿瘤活性采用MTT法测定。结果与讨论 从Aspergillus sp. HDN19-401中分离得到9个化合物，通过对比核磁数据确定为：6-O-methylaverufin(1)、6,8-di-O-methylaverufin (2)、6,8-di-O-methylnidurufin (3)、aversin (4)、oxisterigmatocystin A (5)、oxisterigmatocystin B (6)、fellutamide C (7)、notoamides B (8)、notoamides C (9)，除化合物2外，其余均为首次从极地来源真菌中分离得到，化合物7对K562细胞系具有中等的抑制活性，IC50值为7.78 μmol/L。

Studies on secondary metabolites from the Arctic marine-derived fungus Aspergillus sp. HDN19-401

Abstract Objective To study the secondary metabolites produced by marine-derived fungus Aspergillus sp.HDN19-401 isolated from the Arctic and their biological activities. Methods The fermentation extracts were isolatedand purified by reversed phase silica gel column chromatography, sephadex LH-20 and semi-preparative HPLCand so on. The chemical structures of these compounds were confirmed by ESI-MS and NMR spectral analysis andcomparison with the data of literature. MICs were obtained by the broth microdilution method. Antitumor activity wasstudied by the MTT assay. Results and Conclusion Nine compounds were isolated from the extracts of Aspergillussp. HDN19-401, and were identified as 6-O-methylaverufin (1), 6, 8-di-O-methylaverufin (2), 6, 8-di-O-methylnidurufin(3), aversin (4), oxisterigmatocystin A (5), oxisterigmatocystin B (6), fellutamide C (7), notoamides B (8), andnotoamides C (9). Except compound 2, the other compounds were isolated from Arctic fungus for the first time.Compound 7 showed moderately cytotoxic activity against K562 cell line with an IC50 value of 7.78