| 光辉霉素对胃癌细胞周期、凋亡的影响及可能机制 |
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| 引用本文： | 何灏澜,李倩,姜晗,王晓,陆明.光辉霉素对胃癌细胞周期、凋亡的影响及可能机制[J].临床肿瘤学杂志,2019,24(2):133-136. |
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| 作者姓名： | 何灏澜 李倩 姜晗 王晓 陆明 |
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| 作者单位： | 沈阳医学院附属中心医院消化内科, 110024 沈阳;沈阳医学院附属中心医院消化内科, 110024 沈阳;沈阳医学院附属中心医院消化内科, 110024 沈阳;沈阳医学院附属中心医院消化内科, 110024 沈阳;沈阳医学院附属中心医院消化内科, 110024 沈阳 |
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| 摘 要： | 目的探讨光辉霉素(MTM)对胃癌细胞周期、凋亡的影响及可能的机制。方法体外培养胃癌BGC-823、SGC-7901、MKN-28、AGS和正常胃黏膜GES-1细胞,采用实时荧光定量(QPCR)和Western blotting检测SP1 mRNA和蛋白表达。0、25、50、100 nmol/L MTM处理BGC-823细胞24 h,QPCR和Western blotting检测SP1、p53、p21 mRNA和蛋白表达。流式细胞术检测50 nmol/L MTM处理BGC-823细胞周期和凋亡的变化。结果 QPCR检测胃癌BGC-823、SGC-7901、MKN-28、AGS细胞系中SP1 mRNA表达量为6. 12±0. 15,5. 42±0. 24,3. 33±0. 21,3. 01±0. 12,均显著高于GES-1细胞(P<0. 05); Western blotting检测SP1蛋白表达与mRNA表达一致。0、25、50、100 nmol/L MTM处理BGC-823细胞,SP1 mRNA和蛋白表达逐渐降低,p53、p21 mRNA和蛋白表达逐渐升高。50 nmol/L MTM组SP1表达量最低(mRNA:0. 48±0. 12;蛋白:0. 28±0. 09),p53表达量最高(mRNA:5. 37±0. 45;蛋白:1. 29±0. 20); 100 nmol/L MTM组p21表达量最高(mRNA:4. 92±0. 53;蛋白:0. 86±0. 15);与0 nmol/L MTM组比较,差异具有统计学意义(P<0. 05)。流式细胞术结果显示,50 nmol/L MTM组BGC-823细胞G0/G1比例为(63. 71±2. 14)%和凋亡率为(24. 68±1. 09)%,均明显高于0 nmol/L MTM组(57. 39±1. 83)%和(9. 23±0. 75)%],差异具有统计学意义(P<0. 05)。结论 MTM通过降低SP1、上调p53、p21表达水平来增加胃癌细胞周期阻滞,诱导凋亡。
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| 关 键 词： | 胃癌 SP1 光辉霉素 P53 P21 |
| 收稿时间： | 2018-09-12 |
| 修稿时间： | 2018-12-16 |
Effect of mithramycin on cell cycle and apoptosis of gastric cancer
cells and its possible mechanism |
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| HE Haolan,LI Qian,JIANG Han,WANG Xiao,LU Ming.Effect of mithramycin on cell cycle and apoptosis of gastric cancer
cells and its possible mechanism[J].Chinese Clinical Oncology,2019,24(2):133-136. |
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| Authors: | HE Haolan LI Qian JIANG Han WANG Xiao LU Ming |
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| Institution: | Department of Gastroenterology,Central Hospital Affiliated to Shenyang Medical College,Shenyang,China |
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| Abstract: | Objective To investigate the
effect of mithramycin （MTM） on cell cycle and apoptosis of gastric cancer cells and its
possible mechanism. Methods BGC-823， SGC-7901， MKN-28， AGS and normal gastric mucosa GES-1 cells were cultured in vitro.
Real-time fluorescence quantitative analysis （QPCR） and
Western blotting were used to detect the expression of SP1 mRNA and protein.
BGC-823 cells were treated with 0， 25， 50 and
100 nmol／L MTM
for 24 hours. The expression of SP1， p53 and p21 were detected by QPCR and Western blotting. Flow
cytometry was used to detect the changes of cell cycle and apoptosis in BGC-823
cells treated with 50 nmol／L MTM. Results
The expression of SP1 in BGC-823， SGC-7901， MKN-28
and AGS cell lines was 6.12 ±0.15， 5.42±0.24， 3.33±0.21 and
3.01±0.12 by QPCR， which
were significantly higher than those in GES-1 cell lines （P＜0.05）. Western blotting showed that the expression of SP1 protein was
consistent with that of mRNA. When BGC-823 cells were treated with 0， 25， 50 and
100 nmol／L MTM， the expression of SP1 decreased gradually， while the expression of p53 and p21 increased gradually. Compared
with 0 nmol／L MTM
group， the expression of SP1 was the lowest and p53 was the highest in 50
nmol／L MTM group and the expression of p21 was the highest in 100 nmol／L MTM group（P＜0.05）. Flow
cytometry showed that the G0／G1 ratio
and apoptotic rate of BGC-823 cells in 50 nmol／L MTM group were （63.71±2.14）％ and （24.68±1.09）％ respectively， which
were significantly higher than those in 0 nmol／L MTM group. Conclusion Mithramycin can increase cell cycle arrest
and induce apoptosis by decreasing SP1， increasing p53 and p21 expression. |
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| Keywords: | Gastric cancer SP1 Mithramycin p53 p21 |
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