寻常型银屑病和汗孔角化症患者外周血Vγ9Vδ2 T细胞的表型分析

 目的 比较斑块状寻常型银屑病（psoriasis vulgaris，PV）和汗孔角化症（porokeratosis，PK）患者的外周血Vγ9Vδ2 T细胞比例和表型的变化。方法 采用流式细胞术来检测PV组（n=33）、PK组（n=19）及正常对照（normal control，NC）组（n=33）的外周血单个核细胞（peripheral blood mononuclear cell，PBMC）中CD3、Vγ9、Vδ2、CLA、CD27、CD45RA的表达。结果 与NC组相比较，Vγ9Vδ2 T细胞占总T细胞的比例和CLA⁺Vγ9Vδ2 T细胞占总Vγ9Vδ2 T细胞的比例分别在PV组（t=-2.349，P=0.022 4；t=-3.277，P=0.001 7）和PK组（t=-2.226，P=0.030 6；t=-2.901，P=0.005 5）出现显著减少；Vγ9Vδ2 T细胞分化成T_CM的比例在PV组和PK组分别出现明显下降（t=-4.932，P<0.0001；t=-2.572，P=0.013 1）；同时，T_EMRA的比例在PV组和PK组均出现明显升高（t=3.849，P=0.0003；t=2.236，P=0.0353）；T_EM比例的下降仅在PV组具有统计学意义（t=-3.303，P=0.001 8）。结论 PV和PK患者的外周血Vγ9Vδ2 T细胞的比例和表型具有相似性，该细胞可能迁移至皮肤，参与皮肤的自身炎症应答。

Phenotypic analysis of peripheral Vγ9Vδ2 T cells from patients with psoriasis vulgaris and porokeratosis

Objective To investigate the frequencies and phenotypes of peripheral Vγ9Vδ2 T cells from patients with plaque type psoriasis vulgaris (PV) and porokeratosis (PK). Methods The expression of CD3,Vγ9,Vδ2,CLA,CD27,CD45RA were detected in PBMCs from PV group (n=33),PK group (n=19) and normal control (NC)group (n=33) using flow cytometry. Results Compared with NC group,the percentages of Vγ9Vδ2 T cells among the total T cells and CLA⁺ Vγ9Vδ2 T cells over the Vγ9Vδ2 T cells were significantly decreased in PBMCs from patients with PV group (t=-2.349,P=0.0224; t=-3.277,P=0.001 7) and PK group (t=-2.226,P=0.030 6;t=-2.901,P=0.005 5),respectively; the differentiation of Vγ9Vδ2 T cells into T_CMweresignificantly decreased in both PV and PK groups (t=-4.932,P<0.000 1;t=-2.572,P=0.013 1,respectively);meanwhile,the proportion of T_EMRA was significantly increased in both PV and PK groups (t=3.849,P=0.000 3;t=2.236,P=0.035 3,respectively);only the proportion of T_EMwas significantly decreased in PV group (t=-3.303,P=0.001 8). Conclusion There were similarities in the frequencies and phenotypes of peripheral Vγ9Vδ2 T cells from patients with PV and PK,those cells might be involved in the skin-homing process and autoinflammatory responses.