井冈山地区龙脑樟来源真菌多样性和抗菌活性研究

摘要：目的 探究井冈山地区龙脑樟来源真菌的物种多样性和抗菌活性，为新型抗菌活性天然产物的研发提供菌种资源。方法 采用组织切块及研磨法分离植物来源真菌；依据形态学特征观察、ITS测序对菌株进行鉴定；菌株发酵产物经乙酸乙酯萃取，粗浸膏经琼脂纸片扩散法进行抗菌活性检测。结果 从龙脑樟树的叶片、树皮、种子及树周苔藓中分离纯化出真菌197株，经ITS测序比对归属于29个属，显示出较好的真菌多样性。对真菌室温静置发酵后获得的代谢粗提物进行抗菌活性筛选，发现其中50株真菌的次级代谢产物具有抗菌活性，从菌株JDWHS-7-081发酵产物中获得1个化合物。结论 井冈山地区龙脑樟来源真菌具有丰富的物种多样性，筛选获得的抗菌活性菌株具有潜在的研究价值。

Diversity and antibacterial activity of fungi from the traditional medicinal tree Cinnamomum camphora Chvar. Borneol in Jinggangshan

Abstract Objective This study focused on the diversity and antibacterial activity of fungi isolated from Cinnamomum camphora Chvar. Borneol in Jingganshan, which provided microbial resources for the discovery of new antibacterial natural products. Methods Fungi were isolated by tissue dicing and grinding method. Isolates were identified by morphological analysis and ITS sequencing comparisons. The fermentations were extracted with ethyl acetate, and antibacterial tests were carried out with the paper-disc diffusion method. Results A total of 197 strains of fungi were isolated from the leaves, bark, seeds and epiphytic mosses of Cinnamomum camphora Chvar. Borneol,which belonged to 29 different genera and showed rich fungal diversity. The biological activity assay of the crude extracts obtained after these fungi were fermented at room temperature was conducted, and secondary metabolites from 50 fungal strains showed antibacterial activity. One compound was isolated and identified from strain JDWHS-7-081. Conclusion The fungi from Cinnamomum camphora Chvar. Borneol had great species diversity, and the active strains obtained by screening had potential research