绞股蓝总皂苷对二甲基亚硝胺诱导的大鼠肝纤维化的防治作用

目的:探讨中药组分绞股蓝总皂苷对二甲基亚硝胺(DMN)诱导的大鼠肝纤维化的防治作用。方法:采用腹腔注射DMN诱导大鼠肝纤维化模型。在造模4周后,将造模大鼠随机分为模型组、绞股蓝总皂苷组(200 mg.kg-1)及对照药秋水仙碱组(0.1 mg.kg-1),每组10只,灌胃用药2周后取材。观察检测以下指标:①末次体重、肝脾比值;②肝组织羟脯氨酸含量测定;③肝功能指标:血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)、谷胺酰转肽酶(GGT)活性,血清白蛋白(Alb)、总胆红素(TBiL)含量;④肝组织天狼星红染色及HE染色;⑤肝组织脂质过氧化指标:超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)、谷胱甘肽过氧化物酶(GSH-Px)。结果:模型组出现典型的肝纤维化病理改变,与正常组比较,模型组肝组织Hyp含量、血清ALT,AST,GGT活性及TBiL,MDA含量显著升高,同时血清Alb含量、肝组织SOD活性、GSH含量、GSH-Px活性显著降低;较之模型组,绞股蓝总皂苷肝纤维化程度显著减轻,肝组织羟脯氨酸含量显著降低(P<0.01),与秋水仙碱组效应相当。绞股蓝总皂苷组肝功能指标均有显著改善,同时能显著升高肝组织SOD,GSH-Px活性(P<0.05),降低MDA含量。结论:绞股蓝总皂苷对DMN诱导的大鼠肝纤维化有显著的治疗作用。

Effect of gypenosides on DMN-induced liver fibrosis in rats

Objective:To study the effect of gypenosides on DMN-induced liver fibrosis in rats.Method:A rat liver fibrosis model was established by injecting DMN intraperitoneally.Four weeks later,model rats were randomly devided into three groups:the model group,the gypenosides treated group(200 mg·kg-1) and the colchicine treated group(0.1 mg·kg-1),with 10 specimens for each group.After a 2-week treatment,following parameters were observed:① last body weight,weight ratio between liver and spleen;② content of liver hydroxyproline(Hyp);③ activity of serum alanine aminotransferase(ALT),aspartate aminotransferase(AST),γ-glutamyltransferase(γ-GT),content of albumin(Alb) and total bilirubin(TBiL) in serum;④ liver pathology(Sirius red staining and HE staining);⑤ activity of liver superoxide dismutase(SOD),glutathione reduced(GSH),glutathione peroxidase(GSH-Px) and content of liver maleic dialdehyde(MDA).Result:There were classic liver cirrhosis pathological changes in model groups.Compared with the normal group,liver Hyp content,activity of serum ATL,AST,γ-GT and content of serum TBiL,MDA of model groups significantly increased;content of serum Alb and liver GSH,activity of liver SOD and GSH-Px decreased significantly in model groups.In comparison with the model group,liver cirrhosis remarkable improved in the gypenosides group,content of liver Hyp reduced significantly(P<0.01),which was equal to the colchicine group.Compared with the model group,liver function parameters improved markedly in the gypenosides group;liver SOD and GSH-Px activities significantly increased;MDA content reduced significantly(P<0.05).Conclusion:Gypenosides shows an effect in treating DMN-induced liver fibrosis in rats.