五种天然药物抑制晶状体上皮细胞增殖效应比较

目的：对五种天然药物姜黄素（curcumine，Cur）、榄香烯（elemene，Ele）、三氧化二砷（arsenite trioxide，As2O3）、雷公藤内酯醇（triptolide，Tfi）、莪术（rhizoma curcumae，Rc）抑制重组人碱性成纤维细胞生长因子（recombinant human basic fibroblast growth factor，rhbFGF）诱导的牛晶状体上皮细胞（lens epithelial cell，LEC）增殖效应进行比较。方法：将rhbFGF诱导体外培养的LEC发生增殖，并用Cur、Ele、As2O3、Tri、Rc分别与其共同孵育后，用MTT法检测LEC的活性：用流式细胞术（flow cytometer，FCM）检测LEC增殖细胞核抗原（proliferating cell nuclear antigen，PCNA）表达。结果：增殖组LEC活性、PCNA蛋白表达显著高于对照组（P〈0．01）；Cur组、Ele组、As2O3组、％组、Rc组的LEC活性、PCNA蛋白表达均比增殖组显著降低（P〈0．01）。其中Cur、Ele的作用最强，As2O3的作用次之，Tri作用较弱，Rc作用最弱（P〈0．01）。Cur和Ele的作用相近（P〉0．05）。结论：五种天然药物Cur、Ele、As2O3、Tri、Rc均可抑制LEC增殖。Cur和Ele的作用最佳，有希望成为防治后发性白内障的有效药物。

Comparison of inhibitory effect of five natural medicines on proliferation induced by rhbFGF in lens epithelial cell

AIM： To compare the inhibitory effect of five natural medicines on proliferation induced by recombinant human basic fibroblast growth factor （rhbFGF） in lens epithelial cell （LEC）. METHODS： The proliferations of cultured LEC were induced by rhbFGF, which were incubated with five natural medicines, [curcumine （Cur）, elemene（Ele）, arsenite trioxide （As2O3 ）, triptolide （Tri） and rhizoma curcumae （Re） ], respectively. The LEC activities were detected via methyl thiazolyl tetrazolium （MTT）. The proliferating cell nuclear antigen （PCNA） of LEC were detected via flow cytometer（FCM）. RESULTS： The activity of LEC and the expression of PCNA in proliferation group were higher than those in control group （ P 〈 0.01 ）. Compared with the proliferation group, the activity of
LEC and the expression of PCNA were significantly decreased in group Cur, Ele, As2O3, Tri and Rc （ P 〈 0.01）. The inhibitory effects of Cur and Ele are the strongest. As2O3, in the middle. Tri and Rc are the weakest（P 〈 0.01）, the effects of Cur and Ele are similar. CONCLUSION： The five natural medicines can inhibit the proliferation of LEC induced by rhbFGF. Cur and Ele may become effective medicines of preventing and treating after-cataract.