解脲支原体和人型支原体培养鉴定及药敏追踪分析

目的追踪观察支原体检出率和耐药状况及药敏谱改变情况，以更好地指导临床合理规范使用抗生素。方法对非淋菌性尿道炎（NGU）泌尿生殖道标本进行支原体分离培养鉴定计数及药物敏感试验，试剂采用珠海黑马试剂，按要求进行操作并观察结果，统计方法采用χ^2检验。结果解脲支原体（UU）的男、女检出率分别为44．73％、54．49％；人型支原体（MH）男女检出率分别为3．11％、17．78％。UU耐药率，红霉素63.57％、环丙沙星62．32％、氧氟沙星47.41％、罗红霉素34．91％；MH对多种抗生素呈高耐药性，耐药率红霉素98．01％、阿齐霉素92．43％、罗红霉素86．06％、环丙沙星82．50％。结论女性UU阳性检出率有下降，MH阳性检出率增加；支原体对多种抗生素的耐药性明显增加；药敏试验方便临床医师了解医院耐药谱的变化，并对临床准确合理选用抗生素进行治疗具有比较重要的指导意义。

A track analysis on identification and drug-sensitive test of ureaplasma ureaplycium and mycoplasma huminis

AIM To observe and foUow-up the detective rate,resistance and sensitivity spectnnn of mycoplasma in patients with non-gonococcal urethritis （NGU） in order to quide the better use of antibiotics. METHODS A total of 2 234 cases of urogenital specimens were collected. Mycoplasma was cultured after isolated and identificated. Tnen the number of mycoplasma was observed and the drug-sensitive test was made by biochemical analysis. The statistical methods used χ^2 test. RESULTS The positive ＇rates of ureaplasma ureaplyeium （UU） were 44.73% and 54.49% in male and female patients, respectively. And the positive rates of mycoplasma huminis （MH） were 3.11% and 17.7 % in male and female patients, respectively. The drug resistance rate of UU to erythmmycin （ERY）, eiprofloxacin （CIP）, ofloxaein （OFL） and mxithromycin （ROX） were 63.57 % , 62.32%,47.41% and 34.91%, respectively. The drug resistance of MH to ERY, azithromycin （AZI）, ROX and CIP were 98.01%, 92.43%, 86.06% and 82.50%, respectively. CONCLUSION The positive rate of UU in female patients decreases but that of MH increases. Both UU and MH show increased resistance to antibiotics. And the drug-sensitive test is helpful for doctors to select a reasonable drug in treatment.