| HPLC法测定复方泼尼松龙滴鼻液中主组分的含量 |
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| 引用本文： | 苗彩云,;陈江飞.HPLC法测定复方泼尼松龙滴鼻液中主组分的含量[J].中国临床药学杂志,2009(4):227-230. |
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| 作者姓名： | 苗彩云 ;陈江飞 |
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| 作者单位： | [1]新江省宁波市妇女儿童医院药剂科,宁渡315012; [2]浙江宁波市第一医院药剂科,宁波315010 |
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| 摘 要： | 目的采用HPLC法测定复方泼尼松龙滴鼻液中醋酸泼尼松龙和马来酸氯苯那敏的含量。方法色谱柱为C18，流动相为甲醇一O．02mol·L^-1磷酸二氢钾溶液（含0．2％三乙胺，用H3PO4调pH至3．0）（55：45，V/V），检测波长262nm，流速1mL·min^-1，柱温为室温。结果醋酸泼尼松龙和马来酸氯苯那敏分别在25．2～604．8mg·L^-1（r=0．9999）、25．5—612．0mg·L^-1（r=0．9999）内线性关系良好，平均回收率分别为99．2％（RSD=0．99％）、99．1％（RSD=1．00％）。结论本方法操作方便，准确性、重现性好，可测定醋酸泼尼松龙和马来酸氯苯那敏的含量。
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| 关 键 词： | 高效液相色谱法 复方泼尼松龙滴鼻液 醋酸泼尼松龙 马来酸氯苯那敏 |
Determination of prednisolone acetate and chlorpheniramine maleate in compound prednisolone nasal drops by HPLC |
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| Affiliation: | MIAO Caiyun, CHEN Jiangfei (1 Department of Pharmacy, Ningbo Women and Children＇ s Hospital of Zhejiang Province, Ningbo 315012, China ; 2 Department of Pharmacy, Ningbo NO. 1 Hospital of Zhefiang Province, Ningbo 315010, China) |
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| Abstract: | AIM To establish a method for determination of prednisolone acetate and chlorpheniramine maleate in compound prednisolone nasal drops by HPLC. METHODS The content determination was performed on column C18; the mobile phase consisted of methanol 0.02 mol· L- 1 potassium dihydrogen phosphate solution （ containing 0.2% triethylamine and adjusted to pH 3.0 with phosphoric acid） （55：45, V/V）; the detection wavelength was 262 nm; the flow rate was 1 mL· min^- 1 and the column temperature was room temperature. RESULTS The linear ranges of prednisolone acetate and chlorpheniramine maleate were 25.2 - 604.8 mg·L^- 1 （ r = 0.999 9） and 25.5 - 612.0 mg· ^L- l （ r = 0.999 9）, respectively. The average recoveries were 99.2% （ RSD = 0.99 % ） and 99.1% （ RSD = 1.00% ）, respectively. CONCLUSION The method is simple, accurate, reproducible, and suitable for the determination of prednisolone acetate and chlorpheniramine maleate. |
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| Keywords: | HPLC compound prednisolone nasal drop prednisolone acetate chlorpheniramine maleate |
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