黄芪多糖对大鼠蛛网膜下腔出血后早期脑损伤的治疗作用

目的 探讨黄芪多糖（APS）对大鼠蛛网膜下腔出血(SAH)后引起的早期脑损伤是否具有治疗作用。方法 成年雄性SD大鼠120只，采用颈内动脉刺破法建立SAH模型，随机分为假手术(sham)组、SAH模型(SAH+NS)组、APS给药（SAH+APS）组。SAH模型建立后，各组立即腹腔注射相应溶液，24 h后通过T2加权成像（T2WI）来评估脑内损伤情况；应用Nissl染色法观察神经元特有结构尼氏体的形态学改变；应用免疫组织化学和Western blotting方法检测凋亡因子cleaved-Caspase-3和Bcl-2、Bax蛋白的表达水平。 结果 组织学染色及T2W1结果表明，SAH大鼠应用APS（40mg/kg）可以提高Bcl-2的表达水平（P<0.05），下调cleaved-Caspase-3和Bax的表达水平（P<0.05）；T2WI结果显示，SAH大鼠应用APS（40mg/kg）可以降低脑内损伤程度（P<0.05）。 结论 黄芪多糖可以抑制SAH大鼠海马区神经元的凋亡，对早期脑损伤具有一定的治疗作用。

Therapeutic effect of astragalus polysaccharides on early brain injury after subarachnoid hemorrhage in rats

Objective To investigate whether astragalus polysaccharide(APS) has a therapeutic effect on early brain injury induced by subarachnoid hemorrhage (SAH) in rats.Methods 120 adult male SD rats were randomly divided into sham operation (sham) group,SAH model group (SAH + NS) and APS group (SAH + APS).After the establishment of the SAH model,each group was injected with the corresponding solution immediately.After 24hours,T2 weighted image (T2 weighted imaging,T2WI) was used to evaluate the brain injury;Nissl stainingmethod was applied to observe neuronal specific structure,morphological changes of austenite;Application of immunohistochemistry and Western blottingmethod to detect apoptosis cleaved Caspase-3 and the Bcl-2,Bax protein expression level.Results Histological staining and T2WI result showed that APS (40 mg/kg) could increase the expression of Bcl-2 (P ＜ 0.05),down-regulate the expression of cleaved Caspase-3 and Bax (P ＜0.05) The result showed that APS (40 mg / kg) could decrease the degree of brain injury (P ＜ 0.05).Conclusion Astragalus polysaccharide can inhibit the apoptosis of neurons in hippocampus of SAH rats,and has therapeutic effect on early brain injury.