| 枸橼醛在急性早幼粒细胞白血病细胞系K562中的作用 |
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| 引用本文： | 赵艳秋. 枸橼醛在急性早幼粒细胞白血病细胞系K562中的作用[J]. 解剖学报, 2017, 48(3): 310-315. DOI: 10.16098/j.issn.0529-1356.2017.03.010 |
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| 作者姓名： | 赵艳秋 |
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| 作者单位： | 安徽省合肥市第二人民医院安徽医科大学附属合肥医院广德路院区老年医学科，合肥 230011 |
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| 摘 要： | 目的探讨枸橼醛(citral)在急性早幼粒细胞白血病细胞系K562中的作用。方法 Citral作用于体外培养K562细胞系,分别用Wright-Giemsa染色观察细胞凋亡形态;MTT法计算细胞增殖能力;DNA电泳分析DNA碎片;Annexin V-FITC/PI分析细胞凋亡变化;RT-PCR法检测凋亡通路蛋白Bcl、Bax的表达水平变化;流式细胞术分析线粒体膜电位、Caspase-3和核因子(NF)-κB水平变化。结果 Citral以时间和剂量依赖性方式诱导K562细胞凋亡,citral能够诱导线粒体膜电位减少。证实citral诱发细胞凋亡是依赖线粒体途径。结论 Citral对白血病有潜在的治疗效果,有一定的临床应用前景。
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| 关 键 词： | 枸橼醛 K562 线粒体膜电位 反转录聚合酶链反应 |
| 收稿时间： | 2016-09-27 |
Effect of citral on K562 cell line in vitro |
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| ZHAO Yan-qiu. Effect of citral on K562 cell line in vitro[J]. Acta Anatomica Sinica, 2017, 48(3): 310-315. DOI: 10.16098/j.issn.0529-1356.2017.03.010 |
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| Authors: | ZHAO Yan-qiu |
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| Affiliation: | Department of Geriatric Medicine, the Second People’s Hospital of Anhui Medical University Guangde Road District, Hefei 230011, China |
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| Abstract: | Objective To investigate the effect of citral on the acute early young granulocyte leukemia cell line K562.Methods The apoptosis morphology was observed.Diffusion test,DNA electrophoresis,membrane proteins V-FITC/PI staining and Caspase activation in K562 cells were performed to evaluate the effect of citral on the role of K562.Results Citral induced the apoptosis of K562 cells in a time and dose dependent manner.Citral also induced the decreasing of mitochondrial membrane potential(MMP).Conclusion Citral has a potential effect in leukemia treatment and clinical application. |
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| Keywords: | Citral K562 Mitochondrial membrane potential RT-PCR |
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