内皮型一氧化氮合酶基因G894T多态性与青海省汉族妊娠高血压综合征的相关性

目的探讨内皮型一氧化氮合酶(eNOS)G894T基因多态性与青海省汉族妊娠高血压综合征(PIH)的相关性。方法采用限制性片段长度多态性聚合酶链反应(PCR-RFLP)方法对138名妊娠高血压综合征患者和135名正常孕妇e NOS基因G894T多态性进行分型,并测序验证。结果妊娠高血压综合征组基因型频率野生型(GG)、杂合子型(GT)和突变纯合子型(TT)分别为17.4%、82.6%、0;对照组分别为0、95.6%、4.4%,两组基因型频率分布有差异(P0.05)。妊娠高血压综合征组e NOS等位基因频率G、T分别为58.7%、41.3%;对照组分别为47.8%、52.2%,两组比较差异有统计学意义(P0.05),妊娠高血压综合征组G基因频率高于对照组。结论e NOS基因G894T多态性可能与青海省汉族妊娠高血压综合征有关,G等位基因可能为妊娠高血压综合征的易感基因(OR=1.229,95%CI:1.048~1.441),T等位基因可能为妊娠高血压综合征的保护基因。携带GG基因型的孕妇可作为青海省妊娠高血压综合征的易感人群。

Association between G894T polymorphism of endothelial nitric oxide gene and pregnancy-induced hypertension syndrome in Han group of Qinghai

Objective To investigate the association between the gene polymorphism of endothelial nitric oxide synthase (eNOS) and pregnancy-induced hypertension syndrome (PIH) in Han population of Qing hai.Methods eNOS genotype with polymerase chain reaction-restriction fragment length polymorphism was performed in 138 PIH subjects and 135 normal pregnancy subjects.The mutation was confirmed by DNA sequencing.Results The frequencies of eNOS GG,GTandTTgenotypeswere 17.4 ％,82.6 ％,0 in PIH group and0 ％,95.6 ％,4.4 ％ in control Group.There were differences between the two groups of genotype frequency distribution (P ＜ 0.05).The frequencies of the two alleles G and T were 58.7 ％,41.3 ％ in PIH group and 47.8 ％,52.2 ％ in control group,respectively(P ＜ 0.05).The frequency of G gene in PIH group was higher than the control group.Conclusion The study suggests an association between the G894T polymorphism of eNOS gene and PIH in Han group of Qinghai.G allele may be a susceptible gene to PIH (OR =1.229,95％ CI:1.048 ～1.441)and T allele may be a protective gene to PIH.Pregnant women with GG genotype may be a high susceptible group of pregnancy induced hypertension in Qinghai province.