干扰miR-31表达初期Notch和Hedgehog信号通路相关基因在神经干细胞中的表达变化

目的:研究干扰miR-31表达初期Notch和Hedgehog信号通路相关基因在神经干细胞(NSCs)中的表达变化。方法:利用荧光定量PCR对干扰miR-31表达初期Notch和Hedgehog信号通路相关基因在NSCs中的表达变化进行研究。结果:干扰与过表达miR-31后3 d,NSCs中的Notch信号通路相关基因Notch2的表达均增加,Jag2、Dll3和Hes1等的表达均降低;Hedgehog信号通路相关基因Wnt3的表达均增加,Bmp5与Wnt7a的表达均降低。结论:影响miR-31的表达可引发NSCs发生分化,在此过程中Notch与Hedgehog信号通路中几个基因的表达都产生相应改变,表明miR-31与NSCs分化过程相关。

Changes of expression of Notch and Hedgehog signaling pathway related genes in neural stem cells after interfering with miR-31 expression

Objective: To study the expression of Notch and Hedgehog signaling pathway related genes in neural stem cells after interfering with miR-31 expression in neural stem cells(NSCs).Methods: The expression of Notch signaling pathway and Hedgehog signaling pathway related genes in NSCs was studied by fluorescence quantitative PCR.Results: Three days after interference and over-expression of miR-31,the expression of Notch signaling pathway related gene Notch2 was increased,and Jag2,Dll3 and Hes1 were decreased;the expression of Hedgehog signaling pathway related genes Wnt3 was increased,Bmp5 and Wnt7a were decreased.Conclusion: Influencing the expression of miR-31 expression can induce differentiation of NSCs.In this process,the expression of Notch and Hedgehog signaling pathway related genes is changed accordingly.These show that miR-31 is related to the differentiation of NSCs.