何首乌饮对大鼠睾丸间质细胞类固醇激素合成急性调节蛋白和细胞色素P450胆固醇侧链裂解酶蛋白表达的影响

目的探究何首乌饮影响大鼠睾丸间质(Leydig)细胞类固醇激素合成急性调节蛋白(St AR)和细胞色素P450胆固醇侧链裂解酶(P450scc)蛋白表达的变化。方法选用10~20 d的幼年雄性Wistar大鼠,分离、培养Leydig细胞,然后收集。采用氧化损伤的方法建立Leydig细胞衰老模型。实验分组:间质细胞衰老模型组,何首乌饮处理组,首乌丸处理组,正常组和何首乌饮对照组。结果衰老模型组Leydig细胞St AR和P450scc的免疫组织化学染色强度明显低于正常组和何首乌饮对照组(P0.01)。另一方面,何首乌饮和何首乌丸干预组St AR和P450scc的免疫组织化学染色强度明显高于衰老模型组(P0.05),何首乌饮组好于何首乌丸组。结论何首乌饮可提高睾丸Leydig细胞St AR和P450scc蛋白的表达,提高睾酮的合成能力,从而延缓Leydig细胞衰老。

Effect of Heshouwuyin on protein expression of steroidogenic acute regulatory protein and cytochrome P450 cholesterol side chain cleavage enzyme in Leydig cells of rats

Objective To explore the effect of Heshouwuyin on the steroidogenic acute regulatory protein (StAR) and cytochrome P450 cholesterol side chain cleavage enzyme(P450scc) protein expression in Leydig cells of rats. Methods Juvenile male Wistar rat,10-20 days old, were used to isolate the Leydig cells and cultured, and then collected them. The oxidative damage technique was used to establish Leydig cell aging model. This study had a Leydig aging model, Heshouwuyin and Shouwu pill treatments, and normal and Heshouwuyin control group. Results The intensities of immunohistichemical staining for StAR and P450scc were significantly lower in the Leydig aging model group than those in the normal group and Heshouwuyin control group (P<0.01), and were significantly higher in the Heshouwuyin and Shouwu pill group than that in the aging model group (P<0.05). Heshouwuyin group was better than Shouwu pill group. Conclusion Heshouwuyin can increase the protein expression of StAR and P450scc in Leydig cells, improve the synthesis of testosterone, thus delaying the senescence of Leydig cells.