| 体外培养大鼠胚胎脊髓神经干细胞的增殖和分化 |
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| 引用本文： | 李秀凤,管英俊,于丽,孙瑜婉,李云飞.体外培养大鼠胚胎脊髓神经干细胞的增殖和分化[J].潍坊医学院学报,2008,30(6). |
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| 作者姓名： | 李秀凤 管英俊 于丽 孙瑜婉 李云飞 |
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| 作者单位： | 潍坊医学院组织胚胎学教研室,山东,潍坊,261042 |
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| 基金项目： | 山东省优秀中青年科学家研究奖励基金，山东省卫生系统中青年重点科技人才专项基金 |
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| 摘 要： | 目的探讨大鼠胚胎脊髓神经干细胞的体外培养生长及分化情况,为神经干细胞的临床应用提供实验依据。方法取孕13d SD大鼠胚胎脊髓,在条件培养基中培养获得神经干细胞,应用免疫荧光法鉴定。神经干细胞在含血清的培养基中传代培养,应用免疫细胞化学法检测神经干细胞的分化情况。结果从大鼠胚胎脊髓可获得具有自我增殖分化能力的神经干细胞,培养7d即可获得神经球。用免疫细胞化学和免疫荧光法鉴定干细胞分化,可检测到神经元和星形胶质细胞。结论从大鼠胚胎脊髓能分离获得神经干细胞,并可向神经元和星形胶质细胞方向分化。
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| 关 键 词： | 脊髓 神经干细胞 细胞培养 分化 |
Proliferation and Differentiation of Neural Stem Cells Derived from the Spinal Cord of Embryonic Rats in Vitro |
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| LI Xiu-feng,GUAN Ying-jun,YU Li,SUN Yu-wan,LI Yun-fei.Proliferation and Differentiation of Neural Stem Cells Derived from the Spinal Cord of Embryonic Rats in Vitro[J].Journal of Weifang Medical College,2008,30(6). |
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| Authors: | LI Xiu-feng GUAN Ying-jun YU Li SUN Yu-wan LI Yun-fei |
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| Institution: | LI Xiu-feng; GUAN Ying-jun; YU Li; SUN Yu-wan; LI Yun-fei (Histology and Embryology Department of Weifang Medical Colleage; Weifang 261042; China); |
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| Abstract: | Objective To study the characteristics of proliferation and differentiation of neural stem cells(NSCs)derived from the spinal cord of embryonic rats in vitro and to provide experimental evidences for their clinical application.Methods NSCs were obtained from the spinal cord of embryo at the agel3 days of age which was cultured in certain conditional medium and were identified by immunofluorescence. NSCs were induced to differentiate in the medium containing serum and the differentiated cells were identified... |
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| Keywords: | Spinal cord Neural stem cell Cell culture Differentiation |
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