| 腺病毒介导的ING4基因抑制人脑胶质瘤细胞增殖迁移的机制研究 |
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| 作者姓名： | Zhan LP Li QY Zhang Y Zhang TJ Yuan ZC Lu PS |
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| 作者单位： | 江苏大学附属人民医院神经外科,江苏镇江,212002 |
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| 基金项目： | 镇江市社会发展计划项目(SH2009018) |
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| 摘 要： | 目的:观察ING4对人脑胶质瘤细胞株U251增殖及迁移的影响,并探讨其可能的作用机制.方法:以Ad-INC4及腺病毒空载体转染U251细胞,RT-PCR法检测ING4基因的表达水平,Western blot法检测目的蛋白的表达.用MTT试验检测ING4对于U251细胞增殖的影响,通过Boyden Chamber试验检测其对U251细胞侵袭能力的影响.并用免疫印迹方法检测NGF、TrkA的表达变化,通过pull-down试验检测活性RhoA表达.结果:U251细胞感染Ad-ING4 48 h后,RT-PCR结果显示ING4在U251中过表达,且U251细胞的增殖及迁移能力受到明显抑制.免疫印迹方法显示感染AdING4的U251细胞中TrkA和NGF的表达降低,pull-down试验结果显示活性RhoA表达降低.结论:Ad-ING4可以抑制胶质瘤细胞株U251的增殖和迁移,这一作用可能是通过抑制NGF、TrkA和活性RhoA的表达实现的.
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| 关 键 词： | 生长抑制因子 神经生长因子 酪氨酸蛋白激酶受体 胶质瘤 RhoA |
Mechanism of ING4 mediated inhibition of the proliferation and migration of human glioma cell line U251 |
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| Zhan LP,Li QY,Zhang Y,Zhang TJ,Yuan ZC,Lu PS.Mechanism of ING4 mediated inhibition of the proliferation and migration of human glioma cell line U251[J].Journal of Cellular and Molecular Immunology,2011,27(11):1188-1190. |
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| Authors: | Zhan Li-ping Li Qiao-yu Zhang Yan Zhang Tie-jun Yuan Zhi-cheng Lu Pei-song |
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| Institution: | ZHAN Li-ping,LI Qiao-yu,ZHANG Yan,ZHANG Tie-jun,YUAN Zhi-cheng,LU Pei-song Department of Neurosurgery,The Affiliated People's Hospital of Jiangsu University,Zhenjiang 212002,China |
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| Abstract: | AIM: To investigate the effect of Ad-ING4 on proliferation and migration of glioma cells and explore its probable mechanism.METHODS: U251 were infected with Ad-ING4.ING4 gene expression was evaluated by RT-PCR.MTT assay was adopted to evaluate the effect of ING4 on proliferation of U251;Boyden chamber assay was used to check the effect of ING4 on the migration of U251.In ING4 transfected U251,Western blot was used for detecting NGF and TrkA expression;Pull-down assay was used for detecting active RhoA expre... |
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| Keywords: | ING4 nerve growth factor tyrosine kinase receptor glioma cell line RhoA |
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