| IL-12增强结核病患者中性粒细胞吞噬和杀伤结核杆菌的活性 |
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| 作者姓名： | Jiang LN Yao CY Jin QL He WX Li BQ |
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| 作者单位： | 1. 蚌埠医学院免疫学教研室,安徽 蚌埠233030;蚌埠医学院蚌埠医学院病理生理学教研室,安徽 蚌埠233030
2. 蚌埠医学院免疫学教研室,安徽 蚌埠233030;蚌埠医学院感染与免疫安徽省重点实验室,安徽 蚌埠233030 |
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| 基金项目： | 国家科技重大专项基金课题(2008ZX10003011); 安徽省教育厅省高校省级重点实验室重点科研项目(2004sys008); 安徽省高校科学研究项目(KJ2011Z253); 蚌埠医学院科研课题(BY0903) |
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| 摘 要： | 目的:观察IL-12对结核病患者外周血中性粒细胞(PMN)对结核分枝杆菌(M.tb)吞噬和杀伤功能的影响.方法:结核病患者和正常人外周血与异硫氰酸荧光素( FTTC)标记的M.tb共同孵育不同时间后,用流式细胞术(FCM)检测PMN对M.tb的吞噬率;结核病患者和正常人外周血均加入2'7’二氯二氢荧光素二乙酸酯(DCFH-DA)作用,同时加或者不加M.tb共同孵育不同时间后,用FCM检测PMN的活化率并计算活性氧(ROS)产生量;不同浓度IL-12预先作用外周血后,再用上述方法检测PMN对M.tb吞噬率,活化率及产生ROS的变化.结果:结核病患者和正常人PMN对M.tb吞噬率,PMN活化率和ROS产生均随时间延长而增加,但结核病患者5 min吞噬率(51.82±6.93)％高于正常人(47.20±4.26)％(P＜0.05);IL-12预先作用后,结核病患者和正常人PMN吞噬率、PMN活化率和ROS的产生均随浓度增大而显著增加,两者之间无显著性差异.结论:结核病患者外周血PMN对M.tb的吞噬功能以及ROS产生与正常人无显著性差异,仅早期吞噬率较高.IL-12略增强PMN吞噬和产生ROS的反应能力.
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| 关 键 词： | 结核 中性粒细胞 吞噬 活性氧 IL-12 |
The Enhanceing effect of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils in tuberculosis patients |
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| Jiang LN,Yao CY,Jin QL,He WX,Li BQ.The Enhanceing effect of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils in tuberculosis patients[J].Journal of Cellular and Molecular Immunology,2011,27(11):1191-1194. |
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| Authors: | Jiang Li-na Yao Chun-yan Jin Qi-li He Wen-xin Li Bai-qing |
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| Institution: | JIANG Li-na1,2,YAO Chun-yan1,3,JIN Qi-li1,HE Wen-xin1,LI Bai-qing1,3 1Department of Immunology,2Department of Pathophysiology,Bengbu Medical College,3Anhui Key Laboratory of Infection and Immunity,Bengbu 233030,China |
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| Abstract: | AIM: To explore the effects of IL-12 on phagocytosis and killing of Mycobacterium tuberculosis by neutrophils or polymorphonuclear cells(PMNs) in tuberculosis patients.METHODS: The fresh peripheral blood samples from TB patients and healthy adults were incubated with M.tb labeled with FITC,and the percentages of phagocytosis of M.tb by PMNs was measured by flow cytometry(FCM).The fresh peripheral blood samples were incubated with DCFH-DA,and with or without M.tb for different times,the percentage of activat... |
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| Keywords: | tuberculosis neutrophils phagocytosi ROS IL-12 |
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