| 聚醚醚酮-羟基磷灰石-碳纤维复合材料的体外细胞相容性研究 |
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| 作者姓名： | Liu X Deng C Liu J Li J Sui G |
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| 作者单位： | 中国医科大学附属盛京医院骨科;沈阳医学院奉天医院骨科;中国科学院金属研究所; |
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| 基金项目： | 沈阳市科委基金资助项目(F-10-205-1-48) |
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| 摘 要： | 将大鼠成骨细胞与聚醚醚酮-羟基磷灰石-碳纤维(PEEK-HA-CF)复合材料间接和直接培养,通过CCK-8对复合材料的细胞毒性进行评价;流式细胞仪测定材料对细胞增殖指数的影响;测定复合培养后成骨细胞的碱性磷酸酶活性变化;应用扫描电镜(SEM)观察成骨细胞形态学变化。结果表明PEEK-HA-CF复合材料无细胞毒性,复合材料组胞增殖指数与空白对照组比无差异,3d时高于钛合金组。第7d时,PEEK-HA-CF组的碱性磷酸酶活性升高。SEM下,细胞在复合材料上生长良好。PEEK-HA-CF复合材料具有良好的细胞相容性,有希望成为一种新的骨科植入材料。
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| 关 键 词： | 聚醚醚酮 羟基磷灰石 碳纤维 细胞相容性 |
Research on the extracorporeal cytocompatibility of a composite of HA, carbon fiber and polyetheretherket-one |
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| Liu X,Deng C,Liu J,Li J,Sui G.Research on the extracorporeal cytocompatibility of a composite of HA, carbon fiber and polyetheretherket-one[J].Journal of Biomedical Engineering,2011,28(6):1159-1164. |
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| Authors: | Liu Xueyong Deng Chunbo Liu Jiquan Li Jianjun Sui Guoxin |
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| Institution: | Liu Xueyong1 Deng Chunbo2 Liu Jiquan1 Li Jianjun1 Sui Guoxin3 1(Department of Orthopedic Surgery,Shengjing Hospital Affiliated to China Medical University,Shenyang 110004,China) 2(Department of Orthopedic Surgery,Fengtian Hospital,Shenyang Medical College,Shenyang 110024,China) 3(Institute of Metal Research,Chinese Academy of Science,Shenyang 110006,China) |
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| Abstract: | The present research was to study the biocompatibility of a composite of hydroxyapatite(HA),carbon fiber(CF) and polyetheretherket-one(PEEK) by co-culturing with the osteoblasts in vitro.Cell relative growth(RGR) was used as a quantitative assessment for cytotoxicity of the biomaterials by CCK-8.The proliferation index of the co-cultured cells and ALP activity was measured to study the effect of PEEK-HA-CF composites.Morphological properties of the osteoblast cells in vitro were observed by scanning electro... |
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| Keywords: | Polyetheretherketone(PEEK) Hydroxyapatite(HA) Carbon fiber(CF) Cytocompatibility |
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