| 不同剂量地塞米松对大鼠骨质量的作用 |
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| 作者姓名： | Liu Y Chen Y Zhao H Zhong L Wu L Cui L |
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| 作者单位： | 广东医学院药理教研室;广东天然药物研究与开发实验室; |
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| 基金项目： | 国家自然科学基金资助项目(30973574) |
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| 摘 要： | 观察不同剂量地塞米松(Dex)对大鼠骨质量的影响并探讨其作用机制。31只3月龄SPF级雌性SD大鼠随机分为4组:对照组(saline 7只)、Dex-L组(1 mg/kg 8只)、Dex-M组(2.5 mg/kg 8只)、Dex-H组(5 mg/kg 8只);尾静脉注射Dex,2次/周,8周后处死。取胫骨上段制作不脱钙骨切片进行骨形态计量学分析,取左侧尺骨用于测定钙、磷和羟脯氨酸含量,股骨进行骨密度(BMD)和生物力学检测。结果表明:与对照组相比,Dex各组体重均显著下降;胫骨荧光周长百分率、矿化沉积率、骨形成率等显著降低,骨小梁面积百分数,骨小梁数目显著增加,骨小梁分离度显著减少;Dex-L BMD和Dex-M羟脯氨酸含量明显下降,Dex各组力学参数显著降低。提示:Dex显著抑制骨形成,降低骨转换,随着剂量增加,骨小梁面积百分数增加,BMD和骨生物力学性能下降,同时骨内有机质合成降低,非矿化骨形成增加,骨质量降低。以1 mg/kg的Dex对骨的质量影响最明显,该剂量略增加骨小梁面积百分数,但骨形成、骨生物力学性能、骨内有机质含量明显下降。不同剂量的Dex对骨质量的影响不同,剂量与骨质量之间无比例依赖关系。
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| 关 键 词： | 地塞米松 骨形态计量学 生物力学性能 骨密度 骨质量 |
Effects of different doses of dexamethasone on bone qualities in rats |
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| Liu Y,Chen Y,Zhao H,Zhong L,Wu L,Cui L.Effects of different doses of dexamethasone on bone qualities in rats[J].Journal of Biomedical Engineering,2011,28(4):737-43, 747. |
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| Authors: | Liu Yanzhi Chen Yan Zhao Hua Zhong Liping Wu Lingzhi Cui Liao |
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| Institution: | Liu Yanzhi1,2 Chen Yan2 Zhao Hua1 Zhong Liping1 Wu Lingzhi1 Cui Liao1,21(Department of Pharmacology,Guangdong Medical College,Zhanjiang 524023,China)2(Guangdong Key Laboratory for Research and Development of Natural Drugs,China) |
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| Abstract: | This study was aimed to investigate the effects of different doses of dexamethasone(Dex) on bone quality in rats.Thirty-one SD rats were randomly divided into 4 groups,Control(7 with saline),Dex-L(8 with 1mg Dex./kg),Dex-M(8 with Dex.2.5mg/kg),Dex-H(8 with Dex.5mg/kg),with tail injection,twice per week for 8 weeks.All the rats were killed then.Their proximal tibia were processed into undecalcified sections and measured for bone histomorphometry.The content of Ca2+ and hydroxyproline in their left ulnars wer... |
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| Keywords: | Dexamethasone(Dex) Bone histomorphometric Biomechanical property Bone mineral density(BMD) Bone quality |
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