| Pluronic F-127水凝胶复合骨髓间充质干细胞构建组织工程化脂肪的体外研究 |
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| 引用本文： | Wu H,Deng Y,Yan Y,Quan D,Si M. Pluronic F-127水凝胶复合骨髓间充质干细胞构建组织工程化脂肪的体外研究[J]. 生物医学工程学杂志, 2011, 28(6): 1148-1153 |
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| 作者姓名： | Wu H Deng Y Yan Y Quan D Si M |
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| 作者单位： | 中山大学中山医学院病理生理学教研室;广东医学院生理教研室;中山大学化工学院高分子材料所; |
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| 基金项目： | 广东省科技计划国际合作项目基金资助(2009B050200010); 广东东莞市科技计划项目资助(200910815255); 中山大学实验室开放基金资助项目(KF201037) |
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| 摘 要： | 本实验应用Pluronic F-127水凝胶三维培养大鼠骨髓间充质干细胞(BMSCs),观察其生物相容性,并研究其对BMSCs诱导分化成脂肪细胞的影响。原代培养SD大鼠BMSCs,经诱导分化成脂、成骨和成神经鉴定后,用MTT法检测Pluronic F-127水凝胶对BMSCs的细胞毒性;将P4BMSCs均匀混合于水凝胶中,复合BMSCs的水凝胶经成胶后分别用成脂诱导液和完全培养基培养,观察并记录脂滴、脂肪细胞及脂肪组织形成情况,培养2周后油红O染色鉴定。结果表明BMSCs在Pluronic F-127水凝胶中能较好的黏附、生长及增殖。成脂诱导的水凝胶中BMSCs可分化为脂肪细胞,其中少部分聚集成脂肪细胞团,形成脂肪样组织,并经油红O染色鉴定证实。而仅加入完全培养基的水凝胶内BMSCs则未发生分化。提示BMSCs可在Pluronic F-127水凝胶三维支架中培养并被成功诱导分化为脂肪细胞,利用水凝胶负载BMSCs向脂肪细胞诱导分化可望为组织工程化脂肪提供新的思路,Plu-ronic F-127水凝胶可作为BMSCs治疗体外培养的良好支架。
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| 关 键 词： | 骨髓间充质干细胞 PluronicF-127水凝胶 成脂诱导分化 组织工程化脂肪 |
Adipose differentiation and adipose tissue engineering of bone marrow-derived mesenchymal stem cells using pluronic F-127 hydrogel in vitro |
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| Wu Hongfu,Deng Yubin,Yan Yunfa,Quan Daping,Si Meijun. Adipose differentiation and adipose tissue engineering of bone marrow-derived mesenchymal stem cells using pluronic F-127 hydrogel in vitro[J]. Journal of biomedical engineering, 2011, 28(6): 1148-1153 |
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| Authors: | Wu Hongfu Deng Yubin Yan Yunfa Quan Daping Si Meijun |
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| Affiliation: | Wu Hongfu1,2 Deng Yubin1 Yan Yunfa3 Quan Daping3 Si Meijun1 1(Department of Pathophysiology,Zhongshan Medical School,Sun Yat-sen University,Guangzhou 510080,China) 2(Department of Pathophysiology,Guangdong Medical College,Dongguan 523808,China) 3(Department of Polymer Materials,Institute of Chemical Technology,Sun Yatsen University,Guangzhou 510275,China) |
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| Abstract: | The aim of this study is to investigate the growth and proliferation of bone marrow mesenchymal stem cells(BMSCs) three-dimensionally cultured in Pluronic F-127 gel,in order to explore the cellular compatibility of gel and to investigate the feasibility of BMSCs differentiating into adipocytes in gel.Rat BMSCs were isolated from adult bone marrow,and then cultured and amplified in vitro.The BMSCs derived from the 4th passage were seeded on the scaffolds and incubated in adipogenic stimuli culture to differe... |
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| Keywords: | Bone marrow mesenchymal stem cells(BMSCs) Pluronic F-127 hydrogel Adipogenic differentiation Tissue engineering fat |
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