白藜芦醇抑制C6细胞生长与诱导细胞凋亡的实验研究

目的探讨白藜芦醇（Res）在体外抑制胶质瘤C6细胞生长并诱导其调亡的作用。方法采用不同浓度的Res处理C6细胞,通过MTT法检测Res对C6细胞生长增殖的抑制作用,流式细胞技术检测Res作用后的细胞周期分布变化及诱导细胞凋亡情况,电镜下观察Res作用后的细胞超微结构的改变。结果Res能抑制C6细胞的生长增殖,并呈时间浓度依赖性;同时Res能影响C6细胞的细胞周期分布,使细胞阻滞于G1期;Res可以诱导C6细胞发生调亡的改变;经Res处理的C6细胞可出现细胞超微结构的改变。结论Res明显抑制C6细胞生长并诱导其发生凋亡和细胞周期改变。

Suppressing growth and inducting apoptosis of Resveratrol on C6 cell

Objective To investigate the suppressive effect of Resverol（Res） on proliferation of C6 cell. Methods Res at various concentrations were used to process the C6 cell; the inhibitory action on the growth of C6 cell was evaluated through MTT assay. The cell cycle distribution change and apoptosis induced by Res were examined through flow cytometer （FCM）. In addition, the electron microscope was applied to observe the change of the cell＇s ultra microstructure. Results The experiment showed that Res could suppress the growth and multiplication of C6 cell in a does- and time-dependent manner. Simultaneously Res could effect the C6 cell＇s cycle distribution, block the cell advancing from G1 to S period. In the experiment the C6 cell apoptosis was induced by Res and the ultra microstructure of C6 cell changed. Conclusion Res obviously suppressed the proliferation of C6 cell and induced its apoptosis and change the cell cycle.