| HPLC法测定复方替米沙坦片中氢氯噻嗪与替米沙坦的含量 |
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| 引用本文： | 姚国灿,张杨. HPLC法测定复方替米沙坦片中氢氯噻嗪与替米沙坦的含量[J]. 齐齐哈尔医学院学报, 2012, 33(9): 1191-1192 |
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| 作者姓名： | 姚国灿 张杨 |
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| 作者单位： | 姚国灿 (嘉兴学院生物与化学工程分院,314000) ； 张杨 (嘉兴学院生物与化学工程分院,314000) ； |
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| 摘 要： | 目的建立HPLC法测定氢氯噻嗪与替米沙坦复方制剂的有关物质。方法采用C18(4.6mm×250mm,5μm)色谱柱;流动相:0.05mol/L磷酸二氢钾缓冲液(用磷酸调pH至4.0)梯度洗脱;检测波长272nm;流速1.0ml/min;进样量20μl。结果理论塔板数以氢氯噻嗪计算为3000。结论本方法灵敏、专属、简便、准确,可用于氢氯噻嗪与替米沙坦有关物质及降解产物的检查。
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| 关 键 词： | 氢氯噻嗪 替米沙坦 复方制剂 有关物质 高效液相色谱法 |
Determination of Related Substances in Compound Telmisartan and Hydrochlorothiazide Tablet by HPLC |
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| Affiliation: | YAO Guo-can.(School of Biotechnology and Chemical Engineering,Jiaxing University,Jiaxing,Zhejiang 314000,China.) |
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| Abstract: | Objective To establish HPLC method for the determination of related substances in compound telmisartan and hydrochlorothiazide tablet.Methods The separation was performed on Agilent ZORBAX Eclipse SB-C18 column with a mobile phase consisting of acetonitrile and 50 mmol·L-1 KH2PO4 solution(pH 4.0).Gradient elution was used and the flow rate was 1.0 ml·min-1.The detection wavelength was set at 272 nm.The injection volume was 20 μl.Results The numbers of theoretical plates calculated by hydrochlorothiazide were 3,000.Conclusions The method is specific,sensitive and reproducible for the detection of the determination of related substances and degradation substances in compound telmisartan and hydrochlorothiazide tablet. |
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| Keywords: | Hydrochlorothiazide Telmisartan Compound Related substances HPLC |
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