5'-hydroxycotinine-N-oxide, a new nicotine metabolite isolated from rat urine. |
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| Authors: | G Schepers D Demetriou R Stabbert B Diehl J I Seeman |
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| Affiliation: | INBIFO Institut für biologische Forschung, Cologne, Germany. |
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| Abstract: | 1. 5'-Hydroxycotinine-N-oxide, 5-(3-pyridyl-N-oxide)-5-hydroxy-1-methyl-pyrrolidone-2, was identified as a new in vivo metabolite of nicotine. 2. The new metabolite was isolated from the urine of rats treated with S-nicotine and characterized using chemical and spectrometric methods. 3. 5'-Hydroxycotinine-N-oxide was synthesized and characterized by MS and by infrared as well as 1H- and 13C-NMR spectroscopy. 4. Identity of the new metabolite with synthetic 5'-hydroxycotinine-N-oxide was demonstrated by comparing the MS and 1H-NMR spectroscopy data as well as by co-chromatography of a spiked urine sample. |
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