| 应用间期FISH研究在肾癌石蜡包埋组织中抽提细胞核的染色体异常 |
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| 引用本文： | 邹泓,杨壹羚,李露霞,赵瑾,齐妍,梁伟华,郭长军,李锋. 应用间期FISH研究在肾癌石蜡包埋组织中抽提细胞核的染色体异常[J]. 现代预防医学, 2011, 38(7): 1304-1306 |
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| 作者姓名： | 邹泓 杨壹羚 李露霞 赵瑾 齐妍 梁伟华 郭长军 李锋 |
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| 作者单位： | 1. 新疆石河子人学医学院病理教研室,石河子,832002
2. 天津医科大学附属肿瘤医院乳腺病理室 |
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| 基金项目： | 国家自然科学基金，石河子大学高层次人才科研启动基金，石河子大学"263"青年骨干教师资助项目 |
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| 摘 要： | [目的]应用间期FISH研究从石蜡包埋肾癌组织中抽提细胞核的染色体异常,探讨此方法的可行性。[方法]选择20例不同类型的肾癌(8例乳头状肾癌,4例Xp11.2染色体异位性肾癌,6例肾透明细胞癌,2例肾嗜酸细胞腺瘤)的石蜡包埋组织,抽提间期细胞核,制备单细胞悬液,用CEP17探针检测17号染色体的倍体,验证此方法的有效性。[结果]在全部20例肾癌样本中成功进行了FISH检测,其中,8例乳头状肾癌存在17号染色体的三体,2例肾嗜酸性腺瘤与6例肾透明细胞癌中17号染色体倍体正常,4例Xp11.2易位性肾癌存在17号染色体的3倍与4倍体。[结论]在石蜡包埋组织中抽提间期细胞核,应用FISH检测染色体异常的方法敏感、特异、可行。
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| 关 键 词： | 荧光原位杂交 细胞核抽提 染色体倍体 17号染色体 |
USING INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TO STUDY RENAL CELL CARCINOMA IN EXTRACT NUCLEI FROM PARAFFIN-EMBEDDED TISSUE |
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| ZOU Hong,YANG Yi-Ling,LI Lu-Xia,et al.. USING INTERPHASE FLUORESCENCE IN SITU HYBRIDIZATION TO STUDY RENAL CELL CARCINOMA IN EXTRACT NUCLEI FROM PARAFFIN-EMBEDDED TISSUE[J]. Modern Preventive Medicine, 2011, 38(7): 1304-1306 |
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| Authors: | ZOU Hong YANG Yi-Ling LI Lu-Xia et al. |
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| Affiliation: | ZOU Hong,YANG Yi-Ling,LI Lu-Xia,et al.(Department of Pathology,Medical College,Shihezi University,Xinjiang 832002,China) |
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| Abstract: | [Objective]To use interphase fluorescence in situ hybridization(FISH)to study renal cell carcinoma(RCC)in extract isolated nuclei from paraffin-embedded tissue and investigate its efficacy.[Methods]The efficacy of this method was investigated in 8 papillary renal cell carcinoma(PRCC),4 Xp11.2 translocations RCC(Xp11.2RCC),6 clear cell carcinoma(CCRCC),and 2 renal oncocytoma.Using interphase FISH with CEP17 probe to study chromosomal 17 ploid in extract isolated nuclei from formalin fixed and paraffin embedd... |
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| Keywords: | FISH Nuclei extraction Chromosome ploidy Chromosome 17 |
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